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Comment on “A Role for Immature Myeloid Cells in Immune Senescence”

Alejandro Ferrari, Eliana Cela and Daniel González Maglio
J Immunol December 15, 2011, 187 (12) 6167; DOI: https://doi.org/10.4049/jimmunol.1190067
Alejandro Ferrari
Humoral Immunity Research Institute, Pharmacy and Biochemistry School, National Council of Scientific and Technologic Research, University of Buenos Aires, 1113 Buenos Aires, Argentina
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Eliana Cela
Humoral Immunity Research Institute, Pharmacy and Biochemistry School, National Council of Scientific and Technologic Research, University of Buenos Aires, 1113 Buenos Aires, Argentina
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Daniel González Maglio
Humoral Immunity Research Institute, Pharmacy and Biochemistry School, National Council of Scientific and Technologic Research, University of Buenos Aires, 1113 Buenos Aires, Argentina
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We recently read an article published in The Journal of Immunology by Enioutina et al. (1) and found an experimental procedure we believe should be discussed. In this article, as well as in many others by the same authors—also in The Journal of Immunology and other renowned journals (2, 3)—serum-specific Abs are quantified by ELISA. According to our knowledge, this could be a misconception, given that we believe ELISA cannot be used for that purpose.

Polyclonal antisera raised against the same Ag could differ in both the affinity and the concentration of the specific Abs. The main premise of quantification is that sample and reference standards must share all biological and physicochemical properties because the analyte concentration is the only unknown factor. The authors have not declared what they have used as the “reference standard,” not in the article that gives rise to this letter, nor in the others published before. Considering that the samples are polyclonal antisera, we believe there is no way to construct such a standard curve that allows expressing the results as a concentration of specific Igs. Therefore, we think that the only reliable information that can be obtained from this sort of assay is a “relative potency” value, commonly expressed as titer of specific Abs.

We certainly think that this issue does not affect the quality of the work by Enioutina et al., but we do think that the quantitative aspects deserve further discussion.

  • Copyright © 2011 by The American Association of Immunologists, Inc.

References

    1. Enioutina E. Y.,
    2. D. Bareyan,
    3. R. A. Daynes
    . 2011. A role for immature myeloid cells in immune senescence. J. Immunol. 186: 697–707.
    1. Enioutina E. Y.,
    2. D. M. Visic,
    3. R. A. Daynes
    . 2002. The induction of systemic and mucosal immunity to protein vaccines delivered through skin sites exposed to UVB. Vaccine 20: 2116–2130.
    1. Enioutina E. Y.,
    2. D. Bareyan,
    3. R. A. Daynes
    . 2009. TLR-induced local metabolism of vitamin D3 plays an important role in the diversification of adaptive immune responses. J. Immunol. 182: 4296–4305.

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Print ISSN 0022-1767        Online ISSN 1550-6606