Correction: Dendritic Cell RIPK1 Maintains Immune Homeostasis by Preventing Inflammation and Autoimmunity

Joanne A. O’Donnell, Jesse Lehman, Justine E. Roderick, Dalia Martinez-Marin, Matija Zelic, Ciara Doran, Nicole Hermance, Stephen Lyle, Manolis Pasparakis, Katherine A. Fitzgerald, Ann Marshak-Rothstein and Michelle A. Kelliher
J Immunol April 15, 2018, 200 (8) 3020-3021; DOI: https://doi.org/10.4049/jimmunol.1800240

O’Donnell, J. A., J. Lehman, J. E. Roderick, D. Martinez-Marin, M. Zelic, C. Doran, N. Hermance, S. Lyle, M. Pasparakis, K. A. Fitzgerald, A. Marshak-Rothstein, and M. A. Kelliher. 2018. Dendritic cell RIPK1 maintains immune homeostasis by preventing inflammation and autoimmunity. J. Immunol. 200: 737–748.

In the version of this article originally published, incorrect versions of Fig. 3F and Supplemental Fig. 3B, without data from Ripk1DC KO Mlkl−/− and littermate controls, were inadvertently included. A revised version of Fig. 3 is shown below. The legend for Fig. 3 was correct as published and is shown below for reference.

FIGURE 3.
FIGURE 3.

DC necroptosis underlies the systemic inflammation in Ripk1DC KO mice. (A) DCs from CD11cCre Ripk1fl/D138N (Ripk1DC KO/D138N) and Ripk1DC KO Ripk3−/− mice are protected from necroptosis in vitro. BMDCs were treated with SMAC mimetics and zVAD-fmk. Effects on cell viability were measured at 18 h using Cell Titer Glo. n = 3–6 samples. (B) Spleen and lymph node (LN) weights and lymph node cellularity from CD11cCre, Ripk1DC KO, Ripk1DC KO/D138N, Ripk1DC KO Ripk3−/−, and Ripk1DC KO Mlkl−/− mice. (C) Neutrophil and inflammatory monocyte numbers in the spleens of CD11cCre, Ripk1DC KO, Ripk1DC KO/D138N, Ripk1DC KO Ripk3−/−, and Ripk1DC KO Mlkl−/− mice. (D) H&E and Masson’s trichrome staining of spleens from Ripk1DC KO, Ripk1DC KO/D138N, Ripk1DC KO Ripk3−/−, and Ripk1DC KO Mlkl−/− mice. (E) Neutrophils, inflammatory monocytes, and B cells are elevated in the lymph nodes of Ripk1DC KO and Ripk1DC KO Ripk3−/− mice but not Ripk1DC KO/D138N or Ripk1DC KO Mlkl−/− mice. (F) Proportion of mice serum positive for ANAs at 6 mo (n = 3–6 mice per genotype). Unless otherwise stated, all data shown are from 16 wk-old mice. CD11cCre and Ripk1DC KO mouse phenotyping data shown in (B), (C), (E), and (F) were shown in Fig. 2. Error bars, mean ± SEM. Scale bars, 100 μm. Unpaired two-tailed Student t test (A–C and E). *p < 0.05, **p < 0.01.

Supplemental Fig. 3B has been updated in the supplemental material online. Fig. 3 has also been corrected in the online version of the article, which now differs from the print version as originally published.