Table I. Sequences of crRNA used to target indicated genes and site-specific primers used to amplify amplicons for genotyping

Target	crRNA Sequence	Sequence Primer Forward, 5′-3′	Sequence Primer Reverse, 5′-3′	Clones
NLRC4	`5′-GACGTCTCATGAGCCAGAGG-3′`	`5′-CTAGCTCTGGAGGGTGTGTTCT-3′`	`5′-AATGGAAACCATTTTCTGCAAG-3′`	1, 2
NLRC4	`5′-GGACCAACACCATCACCGCG-3′`	`5′-CCATACCCCATCTTTTCTGAAC-3′`	`5′-GAATTTGAACTTGGTCAGAGCC-3′`	3, 4
NLRP3	`5′-AAGGAAGAAGACGTACACCG-3′`	`5′-TCAGTCTGATTCAGGAGAACGA-3′`	`5′-CTCCTCAAACAGGATTTTCTGG-3′`	1–4
NLRC4/NLRP3	`5′-GACGTCTCATGAGCCAGAGG-3′ (NLRC4)` `5′-AAGGAAGAAGACGTACACCG-3′ (NLRP3)`	`5′-CTAGCTCTGGAGGGTGTGTTCT-3′` `5′-TCAGTCTGATTCAGGAGAACGA-3′`	`5′-AATGGAAACCATTTTCTGCAAG-3′` `5′-CTCCTCAAACAGGATTTTCTGG-3′`	1–4
NAIP	`5′-GACTTGCGTCCTTCAGGAAC-3′`	`5′-AGACTCCCCATAGAAGACCACA-3′`	`5′-CTGTAAAGACAAAGCCAGCCTC-3′`	1–4

All site-specific primers contained a barcode and linker sequence at the 5′ terminus (see text for details), which is not shown.