Table I. Sequences of crRNA used to target indicated genes and site-specific primers used to amplify amplicons for genotyping
TargetcrRNA SequenceSequence Primer Forward, 5′-3′Sequence Primer Reverse, 5′-3′Clones
NLRC45′-GACGTCTCATGAGCCAGAGG-3′5′-CTAGCTCTGGAGGGTGTGTTCT-3′5′-AATGGAAACCATTTTCTGCAAG-3′1, 2
NLRC45′-GGACCAACACCATCACCGCG-3′5′-CCATACCCCATCTTTTCTGAAC-3′5′-GAATTTGAACTTGGTCAGAGCC-3′3, 4
NLRP35′-AAGGAAGAAGACGTACACCG-3′5′-TCAGTCTGATTCAGGAGAACGA-3′5′-CTCCTCAAACAGGATTTTCTGG-3′1–4
NLRC4/NLRP35′-GACGTCTCATGAGCCAGAGG-3′ (NLRC4) 5′-AAGGAAGAAGACGTACACCG-3′ (NLRP3)5′-CTAGCTCTGGAGGGTGTGTTCT-3′ 5′-TCAGTCTGATTCAGGAGAACGA-3′5′-AATGGAAACCATTTTCTGCAAG-3′ 5′-CTCCTCAAACAGGATTTTCTGG-3′1–4
NAIP5′-GACTTGCGTCCTTCAGGAAC-3′5′-AGACTCCCCATAGAAGACCACA-3′5′-CTGTAAAGACAAAGCCAGCCTC-3′1–4
  • All site-specific primers contained a barcode and linker sequence at the 5′ terminus (see text for details), which is not shown.