Table II. Comparison of published CD4 ABC values with data generated in this study
TargetSpecificationsMedian ABCInstrumentAssayReference
CD4+ T lymphocytesCryopreserved47,000Flow cytometerQuantiBrite(25)
Cryopreserved, fixed42,000Flow cytometerQuantiBrite(25)
Cryopreserved, fixed46,000Mass cytometerAtom per cell(25)
Cryopreserved145,000Mass SpectrometerTargeted multiple reaction monitoring mass spectrometry(26)
Fresh51,000Flow cytometerQuantiBrite(28)
Fresh39,000Flow cytometerQIFI Kit(28)
Fresh173,000Flow cytometerQSC(28)
Fresh98,000Flow cytometerQuantiBrite(29)
Cryopreserved, fixed140,677Mass cytometerQSCCurrent study
CD4+ monocytesFresh3,600Flow cytometerQuantiBrite(28)
Fresh4,200Flow cytometerQIFI Kit(28)
Fresh24,000Flow cytometerQSC(28)
Cryopreserved, fixed26,004Mass cytometerQSCCurrent study