Table I. mRNA expression of inflammatory, antiviral, and innate signaling genes in DCs exposed to F-HIV, C-HIV, and CI-HIV
GenePeak (h)F-HIVC-HIVCI-HIV
Inflammatory genes
 IL-66220*3.02.0
 TNF-α637*9.09.0
 CCL31238*107.0
 IL-10243.02.02.0
Antiviral genes
 IFIT368.6*1.31.4
 APOBEC3A67.3*0.90.7
 APOBEC3B241.40.91.2
 APOBEC3G241.30.80.9
 Tetherin242.4*2.9*2.0*
 TRIM5α480.71.21.4
 TRIM22480.71.51.5
Innate signaling genes
 IRF50.81.11.3
 IRF90.80.60.7
 TLR8481.31.61.3
 MyD88482.2*3.3*3.3*
 NOD2481.21.41.7
 MAVS481.32.12.3
  • DCs (1 × 106/ml) were exposed to an MOI of 8 of F-HIV, C-HIV, or CI-HIV or were mock treated for 3, 6, 12, 24, or 48 h and expression of genes associated with inflammatory and antiviral responses and innate signaling was determined by qPCR. Values have been normalized with mock set at 1 and are shown as the mean values of three to six independent experiments.

  • *p < 0.05, upregulation compared with mock, p < 0.05 compared with F-HIV.

  • –, no significant difference in expression between time points.