Table I. Quantification of human monocytes and macrophages in different organs of humanized mice with or without cytokine treatment
Control MiceM-CSF–Treated MiceGM-CSF–Treated MiceIL-4–Treated Mice
TissueCD14+CD14+CD14+CD14+CD14+CD14+CD14+CD14+
CD11b+CD68+CD11b+CD68+CD11b+CD68+CD11b+CD68+
Blood4.9 ± 0.50.6 ± 0.231.6 ± 5.23.7 ± 0.712.5 ± 3.11.6 ± 0.24.0 ± 1.41.7 ± 0.6
Spleen0.7 ± 0.10.3 ± 0.119.7 ± 7.311.7 ± 5.92.9 ± 0.60.7 ± 0.20.5 ± 0.00.3 ± 0.0
Liver5.7 ± 1.31.0 ± 0.056.8 ± 5.930.9 ± 10.224.2 ± 5.510.2 ± 2.43.2 ± 0.71.8 ± 0.9
Lung20.2 ± 1.72.9 ± 0.862.2 ± 9.428.4 ± 6.436.5 ± 0.117.1 ± 0.811.2 ± 2.14.7 ± 0.5
BM16.4 ± 4.03.3 ± 0.719.0 ± 5.28.4 ± 5.217.2 ± 3.53.6 ± 1.15.3 ± 1.02.0 ± 0.4
  • Humanized mice engrafted with CD34+ FL cells from the same donors were either untreated (control) or treated with M-CSF, GM-CSF, or IL-4. Cells from the indicated tissues were stained for human CD45, CD14, plus CD11b or CD68, followed by flow cytometry analysis. Shown are the mean percentages and SEMs of human CD14+CD11b+ and CD14+CD68+ cells among CD45+ human cells in the blood, spleen, liver, lung, and BM. Data shown are from eight mice per group.