Table I. Reported IAV H-2b–restricted epitopes
PeptideSequenceRestrictionElution Time (min)
HA243-250MNYYWTLLKb28.205
HA290–297ECNTKCQTLikely Kb24.308
HA304–311SSLPYQNIDb Kb21.623
HA332–340TGLRNTPSIDb19.597
HA402–409MNIQFTAVKb23.873
HA468–476SQLKNNAKEIDb17.824
HA475–482KEIGNGCFEFDb27.652
M1128–135MGLIYNRMKb23.540
NA46–54TGICNQNIIDb23.042
NA54–62ITYKNSTWVDb21.078
NA181–189SGPDNGAVAVDb18.559
NA335–343YRYGNGVWIDb25.140
NA425–432SSISFCGVKb27.350
NA430–438FCGVNSDTVDb20.160
NP17–25GERQNATEIDb16.601
NP35–42IGRFYIQMKb25.570
NP55–63RLIQNSLTIDb22.889
NP97–105YRRVNGKWMDb19.709
NP217–225IAYERMCNIKb22.384
NP366–374ASNENMETMDb17.998
NS1133–140FSVIFDRLKb27.377
NS2114–121RTFSFQLIKb27.377
PA224–233SSLENFRAYVDb24.706
PA238–245NGYIEGKLKb21.279
PA300–307GIPLYDAIKb26.044
PA463–470VYINTALLKb24.576
PA648–655SLYASPQLKb21.502
PB1141–149TALANTIEVDb22.788
PB1214–221RSYLIRALKb23.737
PB1465–472RFYRTCKLKb20.183
PB1653–660KNMEYDAVKb18.350
PB1703–711SSYRRPVGIKb19.914
PB1F262–70LSLRNPILVDb25.933
PB2196–206CKISPLMVAYMKb26.260
PB2198–206ISPLMVAYMLikely Db26.420
PB2227–234VYIEVLHLKb26.173
PB2358–365GYEEFTMVKb24.443
PB2689–696VLRGFLILKb29.134
  • Peptide sequences, reported MHC class I restriction, and RP-HPLC elution time on an Agilent 1100 instrument are shown. To determine the exact time that each naturally processed and presented IAV peptide elutes from the HPLC, the elution times of the 38 IAV-derived synthetic peptides were first determined (the elution times are indicated in Fig. 3 and Supplemental Fig. 2 as vertical lines). The data indicate distinct elution time points over a range of ∼13 min under the selected running program and solvent gradient. Several peptides were observed to elute at similar time points, such as HA332–340 and NP97–105.