Table II. Comparison of epitope presentation assessed by HPLC elution and BFA kinetics
Epitopeβ1i−/−β5i/β2i −/−β2i −/−
HPLC (%)BFA (%)RatioHPLC (%)BFA (%)RatioHPLC (%)BFA (%)Ratio
NP366–37440674670.06850
PA224–2339029901110050
PB1703–71151000.051880.015580.09
PB1F262–7041582250.0412100
M1128–1352500225123006910009011
NS2114–121751205013325120
  • Peptide production and presentation of six IAV TCD8+ epitopes were measured from wt, β1i−/−, β5i/β2i−/−, and β2i−/− BMDCs and quantified relative to wt half maximal results (which were considered as 100%). Any difference >10-fold between the HPLC fraction titration and BFA kinetics assessment for the same epitope is shown in boldface italic type.