Table I. Effect of ATM inhibition by KU55933 on cytokine production in human moDC
LPS/IFN-γKU 10 μM + LPS/IFN-γ
CytokineMeanSDMeanSDnp ValueMean Fold Change
IL-23 (pg/ml)2,7582,16011,1156,79632<0.000014.9
IL-1β (pg/ml)35685NS1.0
IL-6 (pg/ml)29,50824,94077,096596,6235NS3.4
IL-12 (pg/ml)52,09625,89483,55521,26660.0452.0
IL-27 (pg/ml)7201521,0722955NS1.5
TGF-β (pg/ml)4802601,0978027NS2.2
  • The role of ATM for other cytokines was determined using ELISA for IL-23, IL-1β, IL-6, IL-12, IL-27, and TGF-β. Data from 5–32 donors are shown for moDC treated with LPS/IFN-γ alone or in the additional presence of 10 μM KU55933. The fold change for individual donors is given, and the p value is obtained from biological triplicate experiments.