Table I. Primers used to create CATLR and to clone TLR15
ProductForwardReverse
mCD4a5′-GCTAGCATGTGCCGAGCCATCTC-3′5′-GGCGCGCCCACCCCTCTGGATAAAAC-3′
mTIRconb5′-GGCGCGCCAGTGTTGGATTTTAATAATTCTAC-3′5′-GTCGACTTAACAGCCAGCAATAAGTATCAG-3′
mTIR45′-GGCGCGCCAGTGTTGGATTTTAATAATTCTAC-3′5′-GTCGACTCAGGTCCAAGTTGCCG-3′
cTIR45′-GGCGCGCCCCTGTCAAACTTTGATATGT-3′5′-GTCGACTTACATGAGTTTTATCTCCTC-3′
cTIR155′-GGCGCGCCCAGTAATCTCACACTTCTG-3′5′-GTCGACTCATTCCATCTCAATTACATC-3′
TLR15c5′-ATGAGGATCCTTATTGGGAGT-3′5′-TCATTCCATCTCAATTACATCC-3′
  • a Murine CD4 extracellular domain. Chicken (c) and murine (m) TIR domains were attached to the murine CD4 extracellular domain via the AscI site (GGCGCGCC).

  • b The control plasmid lacking a TIR domain.

  • c TLR15 primers amplified the native TLR15 sequence.