Table I. RvD1, RvD2, and 17R-RvD1 conversion by oxidoreductases
% Conversion
RvD1RvD217R-RvD1
15-PG-dehydrogenase/EOR68 ± 1048 ± 520 ± 8
11β-Hydroxysteroid dehydrogenase18 ± 30 ± 00 ± 0
  • RvD1, RvD2, or 17R-RvD1 (100 ng) were each incubated with recombinant human EOR (0.5 U) or recombinant human 11β-hydroxysteroid dehydrogenase type 1 (2.5 U) for 30 min at 37°C. Reactions were stopped with 2 vol cold methanol, and solid phase extracted and taken for LC-MS/MS (see Materials and Methods).