Table II. The expression profiles of extracellular matrix-related genes in the presence or absence of IL-17A or -17F in the PCR array
SymbolNameFold Change
Upregulated genes by IL-17A
 ITGB2Integrin, β258.32
 MMP16Matrix metalloproteinase 1621.5
 VCAM1VCAM 16.76
 TIMP3Tissue inhibitor of metalloproteinase 35.68
 ITGA8Integrin, α83.35
 CLEC3BC-type lectin domain family 3, member B3.26
 ITGA4Integrin, α42.28
 KAL1Kallmann syndrome 1 sequence2.21
 NCAM1Neural cell adhesion molecule 12.08
 SPARCSecreted protein, acidic, cysteine-rich2.04
 COL16A1Collagen, type XVI, α12.01
Downregulated genes by IL-17A
 ITGA2Integrin, α20.04
 SPP1Secreted phosphoprotein 10.11
 ITGAMIntegrin, αM0.24
 ITGA5Integrin, α50.25
 SELPSelectin P0.29
 ITGB3Integrin, β30.3
 LAMB3Laminin, β30.34
 ITGB4Integrin, β40.39
 ITGALIntegrin, αL0.41
 CDH1Cadherin 1, type 1, E-cadherin (epithelial)0.42
 ITGA3Integrin, α30.43
 CTGFConnective tissue growth factor0.44
 VTNVitronectin0.44
 ITGA6Integrin, α60.48
Upregulated genes by IL-17F
 MMP12Matrix metalloproteinase 12224.1
 MMP16Matrix metalloproteinase 1693.57
 ITGB2Integrin, β239.07
 TIMP3Tissue inhibitor of metalloproteinase 338.27
 CLEC3BC-type lectin domain family 3, member B19
 KAL1Kallmann syndrome 1 sequence11.54
 ITGA5Integrin, α56.36
 COL6A2Collagen, type VI, α25.42
 ECM1Extracellular matrix protein 12.96
 VCAM1VCAM 12.94
 ITGA7Integrin, α72.6
 SPARCSecreted protein, acidic, cysteine-rich2.55
 MMP3Matrix metalloproteinase 32.19
 NCAM1Neural cell adhesion molecule 12.07
 ITGA4Integrin, α72.01
Downregulated genes by IL-17F
 ITGAMIntegrin, αM0.01
 MMP9Matrix metalloproteinase 90.01
 CTNND2Catenin (cadherin-associated protein), δ20.01
 VTNVitronectin0.01
 ADAMTS8ADAM metallopeptidase with thrombospondin type 1 motif, 80.04
 MMP7Matrix metalloproteinase 70.06
 ADAMTS13ADAM metallopeptidase with thrombospondin type 1 motif, 130.06
 MMP10Matrix metalloproteinase 100.07
 SELPSelectin P0.07
 MMP13Matrix metalloproteinase 130.08
 SELESelectin E0.08
 LAMA1Laminin, α10.11
 HAS1Hyaluronan synthase 10.12
 MMP15Matrix metalloproteinase 150.13
 ITGALIntegrin, αL0.14
 MMP8Matrix metalloproteinase 80.15
 ITGB3Integrin, β30.19
 PECAM1Platelet/endothelial cell adhesion molecule 10.21
 CDH1Cadherin 1, type 1, E-cadherin (epithelial)0.23
 MMP11Matrix metalloproteinase 110.29
 SPP1Secreted phosphoprotein 10.31
 LAMA3Laminin, α30.32
 CNTN1Contactin 10.36
 ITGA3Integrin, α30.37
 ITGB4Integrin, β40.38

  • A mixture of equal amounts of mRNAs from three normal fibroblasts was prepared in the presence or absence of IL-17A or -17F, and mRNA expression profile was evaluated using PCR array. The raw Ct was normalized using the mean value of five housekeeping genes. Fold change (1/2the raw Ct of each mRNA − Ct of housekeeping genes) is shown.