Table I. Primers used to assay expression levels of immune cell- and muscle cell-specific genes
GeneAccession NumberaDirection (5′→3′)Amplicon Size (bp)
IL-6NM_031168FwdGAACAACGATGATGCACTTGC154
RevCTTCATGTACTCCAGGTAGCTATGGT
IL-4NM_021283FwdGGATGTGCCAAACGTCCTC126
RevGAGTTCTTCTTCAAGCATGGAG
MCP-1NM_011333FwdGCTCAGCCAGATGCAGTTAAC153
RevCTCTCTCTTGAGCTTGGTGAC
IP-10NM_021274FwdCCTCATCCTGCTGGGTCTG165
RevGTGGCAATGATCTCAACACG
PPIANM_008907FwdGCAAATGCTGGACCAAACAC97
RevTCACCTTCCCAAAGACCACAT
CD206NM_008625FwdGGATTGTGGAGCAGATGGAAG120
RevCTTGAATGGAAATGCACAGA
Mgl-2NM_145137FwdGTTTGCTCTAATTCCTTCCCAGTC169
RevGTCTAAAATGGCTCTTAGGGTGC
FIZZ-1NM_020509FwdGAGACCATAGAGATTATCGTGGA157
RevCACACCCAGTAGCAGTCATC
Arginase-1NM_007482FwdCAATGAAGAGCTGGCTGGTGT153
RevGTGTGAGCATCCACCCAAATG
Arginase-2NM_009705FwdGAAGTGGTTAGTAGAGCTGTGTC120
RevGGTGAGAGGTGTATTAATGTCCG
iNOSNM_010927FwdCTGCAGCACTTGGATCAG124
RevCGTACCAGGCCCAATGAG
TNFαNM_013693FwdCTTCTGTCTACTGAACTTCGGG163
RevCACTTGGTGGTTTGCTACGAC
MyoDNM_010866FwdGAGCGCATCTCCACAGACAG178
RevAAATCGCATTGGGGTTTGAG
MyogeninNM_031189FwdCCAGTACATTGAGCGCCTAC163
RevACCGAACTCCAGTGCATTGC
  • a National Center for Biotechnology Information reference sequence.

  • IP-10, IFN-γ–inducible protein-10.