Table II. Infection-dependent and -independent Ags recognized by human antisera
Infection-Dependent AgsInfection-Independent Ags
  ORFsFunctionFreq. (%)  ORFsFunctionFreq. (%)
CT858CPAF, serine protease, secreted into host cell94pCT03Plasmid-encoded Pgp3 in outer membrane and also host cell96
CT694Hypothetical, T3SS substrate81CT841ftsH, ATP-dependent zinc protease89
CT813Hypothetical, Inc protein79CT443OmcB, outer membrane pr.85
CT089CopN or LcrE, low calcium response E, T3SS SctW76CT101Hypothetical81
CT442CrpA, in outer membrane and also inclusion membrane75CT147Hypothetical, in both organisms and inclusion membrane72
CT529Hypothetical, Inc75CT240RecR, recombination protein65
CT806Insulinase family74CT812PmpD, outer membrane pr.65
CT798GlgA, glycogen synthase64CT119IncA, inclusion membrane pr.65
CT828NrdB, ribonucleotide–diphosphate reductase subunit β55CT067YtgA, solute pr. binding family60
CT228Hypothetical, Inc49CT875Hypothetical60
CT866GlgB, glycogen-branching enzyme47CT695Hypothetical, T3SS substrate60
CT153MAC/perforin family protein41CT681OmpA, MOMP58
CT116Hypothetical, IncE41CT795Hypothetical56
CT022RpmE2, 50S ribosomal pr. L3155
CT456Hypothetical, T3SS substrate55
CT381ArtJ, arginine transport system substrate-binding pr.53
CT414PmpC, outer membrane pr.49
CT823HtrA, serine protease48
CT110GroEL, heat shock protein 6047
CT541MIP, peptidyl-prolyl cis-trans isomerase44
CT603AhpC, thioredoxin peroxidase44
CT542AspS, aspartyl-tRNA synthetase42
  • The 38 C. trachomatis Ags recognized by >40% of the 99 human antisera were divided into two groups based on their reactivity with antisera from immunized mice and rabbits. Fourteen of the 38 Ags were not detected by any of the immunized animals, thus defined as infection dependent (left columns), and the rest of the 24 Ags were detected by the immunized animals, thus defined as infection independent (right columns). The recognition frequency of each Ag by the 99 human antisera is listed under the “Freq.” column. The ORF numbers in bold indicate that the corresponding Ags were analyzed in Fig. 6. Hypothetical proteins are those with unknown function.

  • CopN, chlamydial outer protein N; CrpA, 15-kDa cysteine-rich outer membrane protein; Inc, inclusion membrane protein; OmcB, outer membrane complex protein B; Pmp, polymorphic outer membrane protein; pr., protein; T3SS, type III secretion system.