Table I. Adhesion of leukocyte subpopulation to activated HUVECs in the absence or presence of epinephrine
SubpopulationsCells Prior to Adhesion %aAdherent Fraction %bCoefficient of Adherence %c
MediumdEpinephrinedMediumEpinephrined
PBMC11.6 (1.6)10.7 (1.4)
 T cells63.3 (2.6)31.1 (2.5) 31.2 (2.6)5.6 (0.7)5.1 (0.6)
  Th cells42.3 (2.8)15.0 (2.2) 16.0 (2.3)3.6 (0.3)3.5 (0.4)
a   Naive17.8 (1.3)2.1 (0.7) 2.5 (0.6)0.8 (0.4)0.7 (0.2)
b   CM13.6 (1.1)7.3 (1.1) 7.6 (1.2)5.4 (0.4)5.1 (0.6)
c   EM5.5 (0.3)4.4 (0.5)* 4.6 (0.6)8.1 (0.9)7.9 (1.3)
d   Effector3.4 (0.4)0.5 (0.1) 0.6 (0.1)1.5 (0.4)1.7 (0.3)
  Cytotoxic T cells18.1 (2.1)10.8 (1.6) 10.7 (1.5)6.1 (0.6)5.6 (0.6)
e   Naive11.1 (1.8)2.8 (0.4) 3.1 (0.4)3.0 (0.5)2.8 (0.4)
f   CM3.2 (0.4)3.2 (0.6)3.1 (0.6)9.8 (1.1)9.1 (1.3)
g   EM1.6 (0.2)2.5 (0.4)* 2.4 (0.4)15.6 (1.8)14.0 (2.2)
h    Effector 1.7 (0.4) 1.7 (0.4) 1.5 (0.4) 12.2 (2.5) 9.4 (1.6) *
i   γ/δ T cells 2.5 (0.3) 4.1 (0.5) 3.6 (0.4) * 17.6 (2.2) 13.3 (1.4)
j   NKT-like cells 2.9 (0.4) 3.5 (0.6) 2.9 (0.5) * 13.6 (2.3) 10.3 (1.3) *
 NK cells12.0 (2.0)31.5 (3.1) 28.4 (2.9) 32.6 (4.4)27.1 (3.5)
k  Immunomodulatory0.6 (0.1)0.8 (0.1)0.7 (0.1)14.9 (1.8)12.5 (1.4)
l   Cytotoxic 11.3 (1.9) 30.8 (3.1) 27.7 (2.9) 33.5 (4.4) 27.9 (3.5)
 Monocytes9.4 (1.1)24.2 (1.6) 27.2 (1.9)* 30.1 (3.5)31.5 (3.8)
m  Conventional8.2 (1.0)19.3 (1.8) 21.9 (1.9)* 27.4 (3.4)29.0 (3.5)
n   Proinflammatory 1.3 (0.2) 5.0 (0.5) 5.3 (0.4) 47.6 (3.4) 47.5 (6.4)
  • Rows show mean (± SEM) of different leukocyte subpopulations. The lowercase letters refer to respective subsets in figures.

  • a Proportion of leukocyte subpopulations from total PBMCs prior to adhesion.

  • b Proportion of leukocyte subpopulations from total cells in the adherent fraction after seeding of 5 × 105 PBMCs on activated HUVECs in the absence (medium) or presence of epinephrine (10−8 M, 1832 pg/ml).

  • c Coefficient of adherence indicates the ratio of cell numbers in the attached fraction to the cell numbers prior to adhesion. Note that the subpopulations mobilized by epinephrine in vivo are in boldface type (n = 7).

  • d For pairwise comparisons between adherent cells/cells prior to adhesion and between both experimental conditions (medium/epinephrine)

  • * p < 0.05, p < 0.01, p < 0.001.