Table I. Flow cytometric analysis of splenic cell populations at 6 and 12 mo of age
6 mo12 mo
Sle16Sle16Sle18Sle18B6Sle16Sle16Sle18Sle18B6
Cell PopulationFACS StainingNo. = 7No. = 8No. = 8No. = 7No. = 14No. = 12No. = 8No. = 11
Cells (106/ml)62.18 ± 4.47a38.49 ± 5.6739.99 ± 5.0243.09 ± 3.1392.57 ± 6.99c,aa,bbb52.33 ± 2.7750 ± 2.9338.94 ± 4.77
% B cellsCD19+44.97 ± 2.244.15 ± 1.3645.92 ± 2.4637.49 ± 1.6542.29 ± 1.18b,aaa54.46 ± 1.1846.97 ± 2.0150.28 ± 2.01
 (No. ×106)(27.34 ± 2.48a)(16.99 ± 2.54)(18.29 ± 2.23)(16.37 ± 1.76)(38.8 ± 2.73c,bbb)(28.94 ± 1.95)(23.5 ± 1.75)(19.58 ± 2.56)
% T cellsCD19, CD5+40.09 ± 1.5743.87 ± 1.3342.16 ± 2.7645.72 ± 231.71 ± 1.4828.94 ± 1.69b29.67 ± 1.7136.22 ± 1.85
 (No. ×106)(25.49 ± 1.85)(16.96 ± 2.69)(17.14 ± 2.91)(19.62 ± 1.46)(29.33 ± 2.71c,aa,bb)(15.34 ± 1.23)(14.89 ± 1.25)(13.96 ± 1.77)
% Plasma cellsCD19, CD90, CD138+0.69 ± 0.260.56 ± 0.110.56 ± 0.090.55 ± 0.032.05 ± 0.19bbb,ccc1.35 ± 0.11b0.72 ± 0.070.62 ± 0.09
 (No. ×106)(0.42 ± 0.16)(0.23 ± 0.05)(0.2 ± 0.02)(0.23 ± 0.01)(1.94 ± 0.28a,bbb,ccc)(0.75 ± 0.09bb)(0.36 ± 0.04)(0.21 ± 0.02)
% MZ B cellsCD19gatedCD21highCD23low8.9 ± 1.6411.02 ± 1.1811.99 ± 1.29.2 ± 0.456.25 ± 0.69a,bb9.11 ± 0.566.87 ± 0.68b10.78 ± 0.82
 (No. ×106)(2.61 ± 0.55)(1.99 ± 0.46)(2.12 ± 0.28)(1.51 ± 0.20)(2.4 ± 0.32)(2.66 ± 0.28)(1.59 ± 0.16)(2.21 ± 0.38)
% FZ B cellsCD19gatedCD21lowCD23high63.24 ± 3.7363.13 ± 2.42b62.5 ± 1.18b73.43 ± 0.8469.27 ± 1.37b67.13 ± 1.7468.09 ± 3.7961.4 ± 1.26
 (No. ×106)(17.33 ± 2.68)(10.45 ± 1.28)(11.55 ± 1.57)(12.09 ± 1.39)(26.81 ± 1.86cc,bbb)(19.13 ± 1.08)(16.13 ± 1.8)(11.95 ± 1.54)
% B1a cellsCD19gatedCD5+3.42 ± 0.252.78 ± 0.253.76 ± 0.283.67 ± 0.162.89 ± 0.17aa3.98 ± 0.193.84 ± 0.33.54 ± 0.29
 (No. ×106)(0.91 ± 0.06a)(0.55 ± 0.06)(0.65 ± 0.05)(0.59 ± 0.05)(1.11 ± 0.1b)(1.19 ± 0.13b)(0.9 ± 0.08)(0.66 ± 0.08)
% Activated B cellsCD19gatedCD69+10.22 ± 0.887.96 ± 0.416.95 ± 0.999.14 ± 0.7416.61 ± 0.93bbb,ccc13.78 ± 0.77b,dd8.61 ± 0.319.33 ± 0.52
 (No. ×106)(2.85 ± 0.19a,cc)(1.36 ± 0.21)(1.22 ± 0.23)(1.44 ± 0.10)(6.42 ± 0.61bbb,ccc)(4.02 ± 0.35b,d)(2 ± 0.12)(1.86 ± 0.27)
% Activated T cellsCD4gatedCD69+20.56 ± 2.6bb19.29 ± 0.87b18.69 ± 0.9712.89 ± 0.7834.54 ± 1.41bb35.39 ± 1.8bbb26.34 ± 2.7222.65 ± 1.27
 (No. ×106)(5.86 ± 0.68b)(3.18 ± 0.41)(3.21 ± 0.58)(2.55 ± 0.28)(10.22 ± 1.11a,bbb,cc)(5.5 ± 0.58)(3.79 ± 0.34)(3.14 ± 0.41)
% Naive T cellsCD4gatedCD62L+57.84 ± 3.2660.37 ± 1.4663.37 ± 1.7765.52 ± 1.6128.76e ± 2.23bb,cc41.27 ± 2.4347.57 ± 3.650.03 ± 2.03
 (No. ×106)(14.6 ± 0.98)(10.55 ± 2.16)(11.11 ± 2.14)(12.94 ± 1.14)(8.27 ± 1.17)(6.4 ± 0.65)(7.2 ± 0.96)(7.2 ± 1.08)
% Memory T cellsCD4gatedCD44highCD2528.39 ± 2.528.51 ± 1.4925.74 ± 1.924.04 ± 1.0250.19 ± 1.78bbb48.46 ± 1.86bbb41.64 ± 3.333.4 ± 1.49
 (No. ×106)(7.43 ± 1.11c)(4.59 ± 0.53)(4.11 ± 0.44)(4.67 ± 0.31)(14.66 ± 1.4a,bbb,cc)(7.33 ± 0.58)(6.01 ± 0.45)(4.46 ± 0.43)
% TregsCD4gatedCD25+FoxP3+20.47 ± 1.95bb17.61 ± 0.97bb16.88 ± 0.81b10.17 ± 0.327.83 ± 1.51b27.62 ± 1.11bNot available22.59 ± 1.23
 (No. ×106)(5.24 ± 0.7bb)(2.98 ± 0.4)(2.8 ± 0.37)(2.01 ± 0.2)(8.18 ± 0.92a,bbb)(4.29 ± 0.43)(3.03 ± 0.32)
  • Results are shown as mean ± SEM. The p values were considered significant when <0.05 according to the Kruskal-Wallis test with Dunn’s multiple comparison test. Statistical analysis as follows:

  • a Sle16 versus Sle16Sle18.

  • b Any congenic versus B6.

  • c Sle16 versus Sle18.

  • d Sle16Sle18 versus Sle18.

  • e CD62L staining was available only for six mice. When gated cells are indicated, the percentage refers to the percentage of the gated population.

  • One symbol: <0.05; two symbols: <0.01; three symbols: <0.001.

  • FZ, follicular zone.