Table II.

Number of B cell precursors for ileal IgA plasma cells: minimal coveragea

SampleSequencedProductively Rearranged SequencesNo. of SetsbUnique SequencesMimimal Coverage (%)Estimated No. of B CellscEstimated SD95% CI
P1H25202 sets (1 × 2, 1 × 3)15259467[24; 382]
P1L25221 set (1 × 2)2010231230[33; 1620]
P2H25161 set (1 × 3)1312.5135138[18; 1002]
P2L25222 sets (2 × 2)181911073[31; 401]
P3H25212 sets (2 × 2)17810066[28; 362]
P3L25242 sets (2 × 2)201713288[36; 486]
P450372 sets (1 × 2, 1 × 3)3214373269[91; 1526]
  • a Minimal coverage was calculated in which 100% identical sequences are considered to be derived from the same B cell.

  • b The number of sets are indicated, with the number of sets × the number of members within that set between brackets.

  • c Estimation of the number of B cell precursors giving rise to the IgA plasma cells within one biopsy was done by comparing the number of unique VH sequences with the VH sequences that were obtained for multiple members of the same set of clonally related sequences. The number of B cell precursors giving rise to the IgA plasma cells within one biopsy is estimated by dividing the number of observed unique sequences of different B cell precursors by the sample coverage, with a correction for nonequal class sizes.