IFN-γ mRNA expression at 18 h following LM-OVA infectiona
| CD8+ T cells | Gr-1+ Neutrophils | |
|---|---|---|
| Control | 1.00 ± 0.19 | 1.00 ± 0.06 |
| LM-OVA | 15.97 ± 0.04* | 1115.77 ± 106b |
a C57BL/6 mice were infected with LM-OVA and 18 h later CD8+ T cells and neutrophils were isolated using magnetic beads (see Materials and Methods). CD8+ T cells and neutrophils were also isolated from uninfected (Control) mice. Quantitative real-time PCR was performed and values were normalized to an internal GAPDH control. Numbers represent the means of three replicates ± SE and expressed as fold change vs control mice.
b p < 0.01.