Table I.

IFN-γ mRNA expression at 18 h following LM-OVA infectiona

CD8+ T cellsGr-1+ Neutrophils
Control1.00 ± 0.191.00 ± 0.06
LM-OVA15.97 ± 0.04*1115.77 ± 106b
  • a C57BL/6 mice were infected with LM-OVA and 18 h later CD8+ T cells and neutrophils were isolated using magnetic beads (see Materials and Methods). CD8+ T cells and neutrophils were also isolated from uninfected (Control) mice. Quantitative real-time PCR was performed and values were normalized to an internal GAPDH control. Numbers represent the means of three replicates ± SE and expressed as fold change vs control mice.

  • b p < 0.01.