Table I.

PCR primers and conditions

CytokineForward PrimerReverse PrimerProduct Length in bp
IL-2aATGTACAGGATGCAACTCCTGTCTTCAGTTTCGTAGTAGAGTTGTGATTG458
IL-4bATGGGTCTCACCTCCCAACTGCTTACTCTCTCTCTTTATAAGTTTCACAAGC456
IL-10bATGCCCCAAGCTGAGAACCAAGACCCATACTGTTACTTCTATGCTTTG249
IL-5aTCATCACGCCAAGGAACTCTTGACGAGCAATGAGACGATG229
IFN-γbATGAAATATACAAGTTATATCTTGGCTTTTACGACAAAGCTCCAGCTTCTCGTAG494
TNF-αaGGATGAACACGCCAGTCGCCCGAGTGACAAGCCCGTAGCC468
IFN-αcAATCTCTCCTTTCTCTCCTGACACGGACCCTCCAACAGTCT364
β-actindGTACCCCATCGAGCACGGCATTGGGTCATCTTCTCGCGGTT180
  • a PCR: 30 cycles of 45 s at 94°C, 45 s at 50°C, 1 min at 72°C.

  • b PCR: 35 cycles of 1 min at 94°C, 1 min at 58°C, 1 min at 72°C.

  • c PCR: 30 cycles at 20 s at 95°C, 25 s at 65–50°C, 40 S at 72°C and 10 cycles of 20 s at 95°C, 25 s at 50°C, 1 min at 72°C.

  • d PCR: 30 cycles of 45 s at 94°C, 45 s at 53°C, 1 min at 72°C.