Primers used for amplification of C4BPAand C4BPB prior to direct sequencing
| Exon | Primers 5′–3’ | (Mg) mM | Temp (°C) |
|---|---|---|---|
| C4BPA | |||
| 1 | (F) CAGTGCTGCTTTATTTCTGCTG | 1.5 | 51.2 |
| (R) TCTGCTGAAAACACCTCCAA | |||
| 2 | (F) CACTCCAGGCTGTCATTTCC | 2.0 | 62.7 |
| (R) TTGATGAAGCTGGGTCTCTG | |||
| 3 | (F) TAAGATGCTGTGTCCCAAGG | 1.5 | 51.2 |
| (R) TCTGAGACAACTTTCCAAAGATAGG | |||
| 4 | (F) TGTGTTTCTGGAAGCAGAGGTG | 2.0 | 58.9 |
| (R) ATCCTTTGCTTGAGGACGGGA | |||
| 5 | (F) TGGGAAATGATATCCAAGAACA | 1.5 | 54.2 |
| (R) GGCTTACACTTGACAACTGAGGT | |||
| 6 | (F) TCCCTGTGCATCTTTACAGGTAT | 3.0 | 58.9 |
| (R) TCAGGTGGCCAAATAAATGA | |||
| 7 | (F) AACTGGATTAGCAGTGGCAG | 1.5 | 58.9 |
| (R) CAGTACGGGGTACCTTTAG | |||
| 8 | (F) TGATACATCAGGCCTTGCAG | 1.5 | 58.9 |
| (R) GTCCCCTTCTGGCACTAACA | |||
| 9 | (F) TCTTCATATAAGTTTAATCC | 1.5 | 50 |
| (R) ATTCCAAGAATCATGCAAG | |||
| 10 | (F) TACATGTATTCTGCAATGTGCTAC | 2.0 | 64.7 |
| (R) GCTCTCAGGACACGTCCAC | |||
| 11 | (F) CCGAGACTGTTATCATGTCCTTC | 2.0 | 64.7 |
| (R) ATGCCACCATTTTTAGGGG | |||
| 12A | (F) CTTGTTTTCCCAGCCTCAAC | 1.5 | 62.7 |
| (R) CATGAGCCACACAGAGGATG | |||
| 12B | (F) GAAAATGGCCCTGGAGGTAT | 1.5 | 58.9 |
| (R) TGGCCCAAACAGTAAAACAA | |||
| C4BPB | |||
| 1 | (F) GACTGGTCAACTGGATTTGCATTC | 1.5 | 58.0 |
| (R) AGCTCCTATTGAGTGACGAGCAT | |||
| 2 | (F) GCAAAAAGACATGAGAATGGG | 1.5 | 58.0 |
| (R) GGATGTGAAGGAGTAAAACAGG | |||
| 3 | (F) TGGCCTTTGCTGTGTTG | 1.5 | 55.0 |
| (R) CAGGGATGTGGCAAATGTC | |||
| 4 | (F) AACAGCTGCAATTAGGGGTG | 1.0 | 55.0 |
| (R) CCAAAACAATGCTGCCAAG | |||
| 5 | (F) CCAGTCTCCATTACCCAATTC | 1.0 | 58.0 |
| (R) AGCATATGCCAGGAGTGATG | |||
| 6 | (F) TTACTTAAGCTAGCCTGGTTCTGAGC | 1.5 | 60 |
| (R) ACATGGTGCATTATACAACTG | 1.5 |