Table III.

Expression of cancer-germline and melanocytic differentiation genes in tumor samples from patient LB2586

Before VaccinationAfter Vaccination
Cutaneous MetastasesCutaneous MetastasesCell Cultures
AB2586-MEL.BLB2586-MEL.G
MAGE-A1(+)+++
MAGE-A3++++++++++
MAGE-A4(+)+++
MAGE-A10(+)(+)++
MAGE-C2++++++++
LAGE-1
NY-ESO1LAGE-2
NA17-A++++++++++++
Tyrosinase+++++++++++++
Melan-AMART-1++++++++++++++
gp100++++
  • Cutaneous metastases were resected in August 2002 (before vaccination, A and B) and in October 2003 (after vaccination). Cell cultures were derived from cutaneous metastases resected in June 2003 (LB2586-MEL.B) and October 2004 (LB2586-MEL.G). Gene expression levels were evaluated semiquantitatively using the intensity of the bands obtained by separating PCR products in agarose gel. The results are expressed as +++, ++, +, or (+) if the amount of the PCR-amplified product is equal or greater than that obtained with 1/1, 1/3, 1/9, and 1/27 dilutions of the reference RNA used for each gene. Lower levels of expression are scored negative. Reference RNA is extracted from tumor cell lines expressing the relevant genes.