Table I.

Frequencies of anti-MAGE-3.A1 and anti-MAGE-C2.B44 CD8 T cells and anti-MAGE-3.DP4 CD4 T cells measured in the blood (PBMC)a

200220032004
OctoberDecemberJanuaryAprilJuneOctoberNovemberDecemberFebruaryJuneAugust
CD8 anti-MAGE-3.A1 (clonotype 1)
 MLPC<3.4 × 10−71.1 × 10−65.2 × 10−65.6 × 10−65.5 × 10−62.8 × 10−5
(2.9 × 106)(8.8 × 105)(9.7 × 105)(1.4 × 106)(9.2 × 105)(5.8 × 105)
 α- and β-chains clonotypic PCR<8.3 × 10−77.5 × 10−65.1 × 10−5
(1.2 × 106)(2.8 × 105)(2.8 × 105)
CD8 anti-MAGE-C2.B44 (clonotype A)
 α- and β-chain clonotypic PCR<8.3 × 10−71.5 × 10−54.2 × 10−52.8 × 10−5
(1.2 × 106)(7 × 104)(2.8 × 105)(2.8 × 105)
CD4 anti-MAGE-3.DP4 (clonotype 2)
 Ex vivo tetramer<3 × 10−78 × 10−65.4 × 10−5
(3.5 × 106)(1.2 × 106)(5.5 × 105)
 β-chain clonotypic PCR2.5 × 10−56.6 × 10−5
(1.2 × 106)(1.2 × 105)
  • a Values correspond to frequency among CD8 or CD4 T cells, respectively. The number of CD8 or CD4 T cells analyzed to establish the frequencies are represented in parentheses.