Table I.

Expression of NKG2D ligands by freshly isolated Tregs, TB lysate-expanded Tregs, and their corresponding CD4+CD25 cell fractionsa

NKG2D LigandFresh CD4+CD25+ TregsFresh CD4+CD25 CellsExpanded CD4+CD25+ TregsExpanded CD4+CD25 Cells
ULBP115.3 ± 2.213.9 ± 3.943 ± 7.715 ± 1.5
ULBP217 ± 4.111 ± 1.220.1 ± 3.512.9 ± 0.9
ULBP312 ± 1.89.2 ± 0.421.7 ± 4.513.5 ± 1.3
MICA/B15.6 ± 4.49.5 ± 0.619.9 ± 2.115.4 ± 1.7
Secondary Ab7.7 ± 1.27.26 ± 18.3 ± 2.515.7 ± 6.3
  • a The MFI ± SE are shown for results obtained with six donors.