Table I.

Growth of Ft LVS or LVSΔiglC in the J774A.1 murine macrophage-like cell linea

Ft StrainTime Postinfection (h)
0244872
WT4.8 × 105 ± 7.6 × 104b7.0 × 107 ± 1.3 × 1076.3 × 107 ± 2.2 × 1071.5 × 107 ± 5.0 × 106
LVSΔiglC3.5 × 105 ± 2.3 × 1056.0 × 104 ± 1.7 × 1046.6 × 103 ± 7.2 × 1036.0 × 103 ± 3.0 × 103
  • a A total of 3 × 105 J774A.1 cells were infected for 2 h with either Ft LVS or LVSΔiglC (MOI: 150) prior to treatment with gentamicin for 1 h. Intracellular bacterial burden was analyzed 0–72 h post-gentamicin treatment by lysis of cells with 0.02% SDS-PBS solution and plating 10-fold dilutions on MHA plates.

  • b Data represent the arithmetic mean ± SD of triplicate cultures and were derived from a single representative experiment (n = 2).