Table I.

Tumor frequency and formation of metastases after subcutaneous inoculation of Ge-Hsp70 or Ge-con cells into SCID and SCID/beige mice

Inoculated cellsaTumor frequency (day 24)bTumor frequency (at autopsy)cMetastases (at autopsy)d
SCID Mice
Ge-Hsp7073 % (30/41)73 % (30/41)0 % (0/30)
  Ge-Hsp70-A73 % (16/22)73 % (16/22)0 % (0/16)
  Ge-Hsp70-C74 % (14/19)74 % (14/19)0 % (0/14)
Ge-con86 % (38/44)86 % (38/44)21 % (8/38)
  Ge-TCR-C87 % (20/23)87 % (20/23)25 % (5/20)
  Ge-GFP-B86 % (18/21)86 % (18/21)17 % (3/18)
Ge76 % (35/46)85 % (39/46)18 % (7/39)
SCID/beige mice
Ge-Hsp7088 % (22/25)88 % (22/25)23 % (5/22)
  Ge-Hsp70-A92 % (11/12)92 % (11/12)18 % (2/11)
  Ge-Hsp70-C85 % (11/13)85 % (11/13)22 % (3/11)
Ge-con92 % (22/24)96 % (23/24)22 % (5/23)
  Ge-TCR-C84 % (10/12)92 % (11/12)27 % (3/11)
  Ge-GFP-B100 % (12/12)100 % (12/12)17 % (2/12)
  • a Ge-Hsp70 cells (clones Ge-Hsp70-A and Ge-Hsp70-C), Ge-con cells (clones Ge-TCR-C and Ge-GFP-B), as well as parental Ge melanoma cells were injected subcutaneously into the flank of SCID or SCID/beige mice (1 × 106cells in PBS/animal).

  • b Percentage and number of animals in which primary tumors were palpable at day 24, before the first mice had to be sacrificed.

  • c Percentage and number of animals in which primary tumors were palpable at the time point of section (day 26 to day 36).

  • d Tumor-bearing animals were carefully inspected for metastases during autopsy and the percentage and number of animals with metastases is given.