Table I.

Grading of histological changes observed at the injection site at 1 wk after a single injection and 4 wk after five injections of 0, 70, or 700 μg of coNS3/4A followed by in vivo EP

Vaccine DoseNo. of Animals of Those Tested with the Indicated Histological Alteration (Peak Score)a
Degeneration/necrosisInflammationFibrosisRegeneration
One week/one injection
 0 μg6/8 (++)5/8 (++)6/8 (++)7/8 (+++)
 70 μg7/8 (+++)6/8 (++++)6/8 (++++)7/8 (++++)
 700 μg8/8 (++++)8/8 (++++)8/8 (+++)8/8 (++++)
Four weeks/five injections
 0 μg7/16 (+)6/16bc (+++)11/16 (++)5/16 (+)
 70 μg9/16 (++)12/16c (+++)11/16 (++)7/16 (++)
 700 μg12/16 (+++)14/16b (++)13/16 (++)6/16 (++)
  • a Intensity of changes: ++++, Very severe and extensive; +++, severe; ++, moderate; +, mild.

  • b p < 0.01, Fisher’s exact test and χ2 test.

  • c p < 0.05, χ2 test only.