Table II.

Tcf-4 potential binding sites in HD5 and HD6 promoter region

Original sequence for gel shift (5′→3′)Position
HD5 number
 1stCTTGAGAACAAAGGCAGTC−120→-102
 2ndAGGAGCATCAAAGGGATCT137→-119
 2nd mutAGGAGCGCCAAAGGGATCT−137→-119
 3rdCTCACGCTTTGATGAAAGC−166→-148
 4thAAATTACTTTGATATTCCC−672→-654
 5thTTGATATACAAAGTGCCAA−886→-868
 6thTTATTTTTCAATGAATAAA−1409→-1391
 7thCAAGAGTACAAAGTTTCAG−1641→-1623
 8thCTAGAGTACAAAGGTGGTC−1707→-1689
 9thCACTGTATCAAAGAAGAAT−2311→-2293
HD6 number
 1stAGGAGCATCAAAGGGACAT137→-119
 2ndTTGGAGAACAAAGGAGCAT−148→-130
 3rdTCACACTACAATGAAGCTT−165→-147
 4thATTATTCATTGAAAGCACT−304→-286
 5thTTTACCCTTTGAATGGTCC−759→-741
 6thAGAAAAAACAAAGCACAAA−856→-838
 7thTTGAGCTACAAAGTATTCA−891→-873
 8thTTATATTACAAAGCTACGG−1214→-1196
 9thAAAAAAAACAAAGTTGAAG−1356→-1238
 10thGACACCCTTTGATGTTCTA−2158→-2140
 10th mutGACACCCTTTGGCGTTCTA−2158→-2140
 11thCATGATCATTGAAAGCTGG−2300→-2282
  • The Tcf-4 potential binding sequence is (A/T)(A/T)CAA(A/T)G. The analysis was performed in the HD5 and HD6 promoter region minus 3 kb. For the competitor, the core binding sequence for Tcf-4 was mutated from (A/T) (A/T) CAA (A/T)G→GC CAA (A/T)G. Numbering of binding sites was performed from proximal to distal with respect to transcriptional start site. This order is consistent with the order in Fig. 6. mut, Mutation.