Table I.

Oligonucleotide sequences used in real-time RT-PCR analysis

SenseAntisense
Human
 GAPDH5′-CCAGCCGAGCCACATCGCTC -3′5′-ATGAGCCCCAGCCTTCTCCAT- 3′
 IL-85′- ATGACTTCCAAGCTGGCCGTGGC- 3′5′- TCTCAGCCCTCTTCAAAAACTTC- 3′
 HD55′-GCCATCCTTGCTGCCATTC- 3′5′-AGATTTCACACACCCCGGAGA- 3′
 HD65′- CCTCACCATCCTCACTGCTGTTC- 3′5′-CCATGACAGTGCAGGTCCCATA- 3′
 Tcf-45′-ATCGTCCCAGAGTGATGTCG- 3′5′-CGGGCCAGCTCGTAGTATTT- 3′
 β-Catenin5′-GCCAAGTGGGTGGTATAGAG- 3′5′-GCTGGGTATCCTGATGTGC- 3′
Mouse
 GAPDH5′- TCATCAACGGGAAGCCCATCAC- 3′5′- AGACTCCACGACATACTCAGCACCG- 3′
 β-Actin5′- GCTGAGAGGGAAATCGTGCGTG-3′5′-CCAGGGAGGAAGAGGATGCGG- 3′
 P-lysozyme5′- GCCAAGGTCTACAATCGTTGTGAGTTG- 3′5′- CAGTCAGCCAGCTTGACACCACG- 3′
 CRS-1C5′- CACCACCCAAGCTCCAAATACACAG- 3′5′- ATCGTGAGGACCAAAAGCAAATGG- 3′
 Crypt-15′- TCAAGAGGCTGCAAAGGAAGAGAAC- 3′5′- TGGTCTCCATGTTCAGCGACAGC- 3′
 Crypt-45′- CCAGGGGAAGATGACCAGGCTG-3′5′- TGCAGCGACGATTTCTACAAAGGC- 3′
 NOD-25′- CGACATCTCCCACAGAGTTGTAATCC- 3′5′- GGCACCTGAAGTTGACATTTTGC- 3′
  • In all cases, control plasmids were cloned to allow assessment of absolute mRNA copy numbers.