Table IIA.

(Continued)

Swiss-Prot Accession No.aProtein IdentificationaColostrumbMature MilkbFunctioncCell Types and Referencesd
S1P1S2P2S1P1S2P2
(P01834)eIg κ-chain C region351422562652Immunoglobulin
(P01842)eIg λ-chain C regions281693Immunoglobulin
(P01871)Ig μ-chain C region13061106Immunoglobulin
(P04220)Igμ heavy chain disease protein (BOT)583354Immunoglobulin
(P23229)eIntegrin α-6 precursor (VLA-6, CD49f)401371Hemidesmosome structureIEC/Mov/DC
(P07498)eκ-Casein precursor664885Micelle formation
(Q08431)eLactadherin precursor (MFG-E8)67229917324771479530Cell bindingDC/Mov/IEC
(P02788)eLactotransferrin precursor1483130553194434918Antimicrobial, iron binding
(P06858)eLipoprotein lipase precursor (LPL)7717219621052Enzyme
(P07195)el-Lactate dehydrogenase B chain (LDH-B)872322411893Enzyme
(O95573)eLong-chain fatty acid CoA ligase 31013564Lipid synthesis
(P14174)eMacrophage migration inhibitory factor (MIF)561882Cell binding
(Q14764)Major vault protein (MVP)1083452Not knownDC/IEC
(Q8N9U0)eMembrane targeting tandem C2 domain containing protein 17421133Not known
(P26038)eMoesin (membrane-organizing extension spike protein)1003586135413510Cytoskeleton membrane interactionsIEC/MC/Mov/B/DC
(P15941)eMucin-1 precursor (MUC-1)571984Cell binding
(O00159)eMyosin Ic (Myosin I beta)822494Intracellular motilityMC/DC
(P62937)ePeptidyl-prolyl cis-trans isomerase A (rotamase) (cyclophilin A)94561512841686Protein biosynthesisDC/Mov
(Q06830)Peroxiredoxin 1 (thioredoxin peroxidase 2)341342251281Redox regulating
(P01833)ePolymeric-immunoglobulin receptor precursor (PIGR)1253144517231515IgA and IgM binding
(P07737)eProfilin-1 (profilin I)461532341625CytoskeletonMov
(P50395)eRab GDP dissociation inhibitor β1224812Signaling
(P35241)eRadixin85373651215711CytoskeletonDC
(P46940)eRas GTPase-activating-like protein IQGAP1502301421292Cell signaling/cell division control
(Q13228)eSelenium-binding protein 1140413273911426Not known
(O95436)eSodium-dependent phosphate transport protein 2B3711011Phosphate transport
(O00560)eSyntenin-1124314665922265Cytoskeletal/signalingDC
(O60603)eToll-like receptor 2 precursor (CD282)3311066LPS-response
(P09758)eTumor-associated calcium signal transducer 2 precursor411331Putative growth factor receptor
(P62988)eUbiquitin621602Protein degradationDC
(P47989)eXanthine dehydrogenase/oxidase7542610794096932159765Enzyme
  • a The Mascot algorithm was used to identify the protein names and the Swiss-Prot accession nos. (www.expasy.org/sprot/).

  • b Colostrum and mature milk were analyzed from two individuals indicated by the numbers 1 and 2 in subscript. Only proteins identified in both individuals are shown. Mascot score (S) cut-off was 25 and numbers of matching peptides (P) are indicated. Mass tolerance of 0.3 Da was used for both MS and MS/MS spectra, and the modifications of carbamidomethylated cysteine and oxidized methionine as well as one missed cleavage were allowed.

  • c Suggested functions are derived from the Swiss-Prot database.

  • d Cell types and references (in parentheses): Mast, Mast cell (43 ); B, B cell (44 ); Mov, immortalized neuroglial cell (45 ); MC, mesothelioma cell (46 ); IEC, intestinal epithelial cell (9 , 47); and DC, dendritic cell (48).

  • e Indicates additional confirmation using the ProID algorithm at confidence levels typically >90 but with cutoff at confidence of >50.