Table II.

TCR CDR3β repertoire in single-cell-sorted Kd-V7+ CD8+ T cells in female NOD mice

DiseaseCDR3βPBLaIsletsaPLNa
#1NondiabeticSDPGNTL15 (68.2%)14 (42.4%)b22 (75.8%)d
SDPRNTL08 (24.2%)b6 (20.7%)
SGDNYEQ2 (9.1%)3 (9.1%)1 (3.4%)
SDPKYEQ2 (9.1%)5 (15.2%)0
SLGDWGYEQ1 (4.5%)00
SPGDTGQL1 (4.5%)00
GDARDWGGRDTQ1 (4.5%)00
SDTKNTL01 (3%)0
SDPENTL01 (3%)0
RTGGSQNTL01 (3%)0
Total223329
#2DiabeticSDSQNTL10 (38.5%)23 (65.7%)b10 (43.5%)
KGSSYEQ6 (23.1%)5 (14.3%)8 (34.8%)
STDWGYEQ3 (11.5%)00
SGDKYEQ3 (11.5%)00
SDFAEQ1 (3.8%)1 (2.9%)0
SSDRFEQ1 (3.8%)3 (8.6%)4 (17.4%)
SSGTDYEQ1 (3.8%)00
SPQGWEQ1 (3.8%)00
SETVYEQ01 (2.9%)0
SEGQKYTL02 (5.6%)1 (4.3%)
Total263523
#3NondiabeticSTDWGYEQ17 (77.3%)1 (5.6%)b1 (4%)c
NRDSAETL3 (13.6%)3 (16.7%)6 (24%)
SQVWGASQNTL1 (4.5%)00
SDRGSSAETLY1 (4.5%)00
SAERGANSDYT011 (61.1%)b2 (8%)d
SDPGNTL01 (5.6%)4 (16%)
SDSQNTL01 (5.6%)5 (20%)
SHRDNYEQ01 (5.6%)1 (4%)
SDAQYEQ003 (12%)
AGDSYEQ002 (8%)
GQSSYEQ001 (4%)
Total221825
#4DiabeticSDAQNTL12 (100%)36 (100%)23 (100%)
Total123623
#5DiabeticSDPAYEQ8 (57.1%)11 (55%)7 (33.3%)
SGDDYEQ3 (21.4%)7 (35%)7 (33.3%)
SDPGNTL3 (21.4%)2 (10%)2 (9.5%)
SSGTDYEQ001 (4.8%)
SDGLYTL003 (14.3%)
SSDWGYEQ001 (4.8%)
Total142021
#6NondiabeticSSDTYEQ34 (100%)29 (90.6%)ND
SSLEYKYEQ02 (6.3%)
SNDTYEQ01 (3.1%)
Total3432
#7NondiabeticSSDTYEQ30 (96.8%)32 (97%)ND
SNDTYEQ1 (3.2%)0
SSWDSSYEQ01 (3%)
Total3133
#8NondiabeticSSLDRVEQ5 (35.9%)0ND
SSGPGQNSDYT3 (21.4%)0
SSRATGGDTGQLY3 (21.4%)0
SSRDWGGADEQ1 (7.1%)0
SSGDNYEQ1 (7.1%)9 (36%)
SSWTGTNERLF1 (7.1%)0
PARDTKNTL01 (4%)
SSDPGMNTL01 (4%)
SSDTKNTL04 (16%)
SSDPGNTL02 (8%)
SSDDTYEQ01 (4%)
TGDNSYEQ02 (8%)
SSDNLYEQ03 (12%)
SSDDTYEQ01 (4%)
SSGDNYKQ01 (4%)
Total1425
  • a Represents the number of TCR sequences identified using the respective CDR3β in single-cell-sorted Kd-V7+ CD8+ T cells in five individual mice.

  • b p < 0.05, PBL vs islet;

  • c p < 0.01, PBL vs PLN;

  • d p < 0.02, islets vs PLN; Fisher’s exact test. Percentage of CD8+ T cells staining with Kd-V7 for PBL, islets, and PLN, respectively, were as follows: mouse #1, 0.43, 0.9, and 0.2%; mouse #2, 0.82, 1.61, and 0.4%; mouse #3, 0.6, 1.2, and 0.5%; mouse #4, 1.5, 2.2, and 0.5%; mouse #5, 4.4, 5.94, and 1.6%; mouse #6, 1.3 and 4.9%, ND; mouse #7, 0.9 and 3.7%, ND; mouse #8, 1.1 and 5.4%, ND.