Table IV.

Evaluation of drug interference in the detection of anti-panitumumab mAbs by ELISA and Biacore assay

Hybridoma Clone DesignationAb Concentration (μg/ml)Concentration of Drug Tolerateda (μg/ml)Molar Excess of Drug ToleratedKD (M)
ELISA drug tolerance
 2.2.10.06424.3380.08.4 × 10−10
 3.7.10.06415.0234.23.2 × 10−9
 18.23.96137.434.73.8 × 10−8
 19.163.98472.17.46.5 × 10−7
 12.246.362157.746.51.1 × 10−6
Biacore assay drug tolerance
 2.2.140.013.80.38.4 × 10−10
 3.7.18.04.80.63.2 × 10−9
 18.22.62.10.83.8 × 10−8
 19.18.049.06.16.5 × 10−7
 12.219.036.01.91.1 × 10−6
  • a Concentration of drug reducing signal in the assay to the threshold value.