Table II.

Affinity and dissociation rate of peptide/HLA-DR1 complexes simultaneously substituted at minor and intermediate solvent accessibility residues

ComplexKD (nM)t1/2 (min)
DR1/P2 V→S80.9 ± 43256 ± 97
DR1/P10 A→G131.4 ± 63693 ± 54
DR1/P3 K→D265.4 ± 122732 ± 207
DR1/P2,10 VA→SG310.5 ± 15887 ± 146
DR1/P3,10 KA→DG4010.2 ± 187531 ± 64
DR1/P2,9,10 VLA→SSG4300 ± 133348 ± 38
DR1/P1,2,10 YVA→VSG25.2 ± 0.9 × 103ND
DR1/P1,2,9 YVL→VSS68.8 ± 2.7 × 103ND
DR1/P3,9,10 KLA→DSG109.3 ± 4.1 × 103ND
DR1/P1,3,9 YKL→VDS1.423 ± 0.06 × 106ND
DR1/P1,3,10 YKL→VDG1.486 ± 0.05 × 106ND
  • ND, not done.