Table I.

Affinity and dissociation rate of substituted peptide/HLA-DR1 complexes

ComplexKD (nM)t1/2 (min)
DR1/HA 306–31870.1 ± 33832 ± 145
DR1/P1 Y→L81.4 ± 33070 ± 216
DR1/P1 Y→V228.6 ± 182737 ± 111
DR1/P9 L→S189.3 ± 101252 ± 84
DR1/P9 L→A208.2 ± 142156 ± 52
DR1/P6 T→K8659.4 ± 312ND
DR1/P1,9 YL→LA250.8 ± 213070 ± 227
DR1/P1,9 YL→VA560.8 ± 271250 ± 39
DR1/P1,9 YL→LS1003.5 ± 56512 ± 27
DR1/P1,9 YL→VS3086.2 ± 124166 ± 15
DR1/P6,9 TL→KA15.7 ± 0.7 × 103ND
DR1/P6,9 TL→KS43.2 ± 1.6 × 103ND
DR1/P1,6 YT→LK1.46 ± 0.06 × 106ND
DR1/P1,6 YT→VK2.58 ± 0.15 × 106ND
DR1/P1,6,9 YTL→LKA1.27 ± 0.08 × 106ND
DR1/P1,6,9 YTL→LKS5.99 ± 0.34 × 106ND
DR1/P1,6,9 YTL→VKA2.26 ± 0.12 × 106ND
DR1/P1,6,9 YTL→VKS7.61 ± 0.35 × 106ND
DR1/P6 T→A67.8 ± 44338 ± 138
DR1/P1,6 YT→LA98.5 ± 53267 ± 168
DR1/P1,6 YT→VA121.8 ± 62857 ± 129
DR1/P6,9 TL→AA101.5 ± 82712 ± 69
DR1/P6,9 TL→AS179.1 ± 122094 ± 106
DR1/P1,6,9 YTL→LAA147.0 ± 112212 ± 49
DR1/P1,6,9 YTL→VAA155.6 ± 152058 ± 65
DR1/P1,6,9 YTL→LAS163.8 ± 81849 ± 97
DR1/P1,6,9 YTL→VAS191.4 ± 71788 ± 48
  • ND, not done.