Effect of various recombinant cytokines on CTL AICDa
| Cytokine | Concentration | % AICD Inhibitionb | pc |
|---|---|---|---|
| IL-4 | 15 ng/ml | 5.0 ± 7.7 | 0.32 |
| IL-10 | 10 ng/ml | 11.8 ± 17.2 | 0.31 |
| IL-12 | 0.1 ng/ml | 13.4 ± 13.7 | 0.43 |
| IL-15 | 10 ng/ml | 2.5 ± 19.1 | 0.46 |
| IFNγ | 100 IU/ml | 7.3 ± 13.5 | 0.39 |
| TGFβ | 10 ng/ml | 11.2 ± 15.1 | 0.16 |
| RANK | 1 ng/ml | 1.9 ± 7.1 | 0.54 |
| LTα1β2 | 25 ng/ml | −6.0 ± 11.3 | 0.22 |
| LTα2β1 | 1 ng/ml | 7.7 ± 15.5 | 0.27 |
a Kb-OVA257–264 monomers were coated onto 24-well plates at 1 μg/ml for 2.5 hours at 37°C. Previously activated CD8 T cells from OT-1 mice were preincubated with medium alone or with the indicated cytokines for 2 h before overnight incubation on the Kb-OVA257–264-coated plate. Cells were then stained with Annexin-V and 7-AAD and analyzed as described. Each cytokine was tested in at least four separate experiments. This table summarizes data from multiple experiments. Numbers represent the average of two separate experiments ± SD of the mean.
b Percentage inhibition of CTL AICD has been calculated to allow comparison between different experiments.
c Values of p were calculated using Student’s t test