Table I.

Summary of expression of surface and intracellular antigens and trafficking receptors by FOXP3+ T cell subsets in three different tissue sourcesa

Neonatal Blood FOXP3+ Cells (n = 3)Tonsil FOXP3+ Cells (n = 3–5)Adult Peripheral Blood FOXP3+ Cells (n = 5–6)
CD45RA+CD45RACD45RA+CD45RACD45RA+CD45RA
T cell activation and differentiationCD45RO1.9 ± 1.298.1 ± 0.56.4 ± 1.496.4 ± 1.733.6 ± 16.597.0 ± 1.2
(11.3 ± 2.1)(387 ± 11.7)(57.1 ± 18.0)(454.0 ± 171.1)(259 ± 55)(643 ± 181)
CD45RB99.7 ± 0.0375.9 ± 7.499.5 ± 0.129.3 ± 3.096.4 ± 1.924.9 ± 6.5
(756 ± 85.0)(360 ± 40.0)(522.4 ± 160.8)(169.7 ± 49.3)(912 ± 198)(223 ± 68)
CD693.9 ± 0.49.0 ± 0.822.3 ± 2.585.6 ± 0.24.6 ± 2.60.8 ± 0.3
(11.1 ± 2.7)(20.0 ± 3.4)(187.6 ± 13.1)(416.3 ± 21.8)(18.9 ± 14.0)(5.9 ± 2.2)
Regulatory T cell associatedCD152b76.9 ± 5.396.7 ± 0.50.6 ± 18.592.5 ± 2.369.6 ± 11.990.6 ± 3.5
(CTLA4)b(50.6 ± 5.2)(299 ± 4.6)(260.1 ± 108.4)(497.9 ± 162.1)(101 ± 38.0)(125 ± 27.1)
CD2593.9 ± 3.991.4 ± 3.293.4 ± 3.593.9 ± 0.886.5 ± 3.083.3 ± 4.3
(164 ± 24.5)(232 ± 3.7)(78.4 ± 12.2)(157.9 ± 6.4)(175 ± 66)(240 ± 96)
AdhesionCD49f16.9 ± 4.134.2 ± 6.020.5 ± 7.412.3 ± 2.971.1 ± 13.769.0 ± 8.4
(VLA6)(13.3 ± 0.2)(18.6 ± 0.3)(12.9 ± 2.2)(8.2 ± 1.1)(47.5 ± 14.5)(29.7 ± 5.3)
CD5823.1 ± 11.186.7 ± 5.56.1 ± 0.780.2 ± 5.555.9 ± 16.191.8 ± 2.9
(LFA-3)(17.3 ± 4.8)(48.8 ± 6.4)(11.0 ± 1.5)(30.4 ± 0.4)(57.7 ± 21.3)(47.0 ± 6.0)
CD100.5 ± 0.11.6 ± 0.51.6 ± 0.60.8 ± 0.14.9 ± 12.80.6 ± 0.3
(1.6 ± 0.8)(3.3 ± 1.1)(2.7 ± 0.4)(3.0 ± 0.1)(8.2 ± 4.7)(2.7 ± 0.6)
CD62L73.8 ± 7.066.7 ± 3.095.0 ± 1.253.6 ± 11.697.1 ± 0.582.3 ± 2.4
(38.1 ± 8.5)(44.9 ± 3.7)(85.0 ± 57.1)(36.6 ± 25.0)(106 ± 21.8)(67.7 ± 11.1)
α4β763.3 ± 2.234.3 ± 4.551.1 ± 0.019.4 ± 2.940.6 ± 10.911.6 ± 2.7
(40.6 ± 4.9)(29.7 ± 4.0)(32.7 ± 3.0)(11.0 ± 3.2)(48.0 ± 20.8)(21.6 ± 5.3)
CLA1.2 ± 0.41.4 ± 0.31.5 ± 0.24.1 ± 0.91.6 ± 0.537.4 ± 6.4
(6.3 ± 2.4)(10.1 ± 4.3)(5.2 ± 0.5)(15.2 ± 2.5)(3.3 ± 0.3)(21.3 ± 3.9)
CD4482.0 ± 2.092.5 ± 0.581.6 ± 9.683.4 ± 7.582.7 ± 16.898.4 ± 1.2
(74.0 ± 2.6)(103 ± 7.7)(24.4 ± 2.4)(27.0 ± 3.4)(62.5 ± 30.5)(46.3 ± 18.2)
CostimulationCD1345.2 ± 2.716.9 ± 3.15.8 ± 0.634.0 ± 8.31.4 ± 0.71.2 ± 0.9
(4.6 ± 1.2)(8.1 ± 1.7)(6.0 ± 0.5)(12.5 ± 1.1)(5.7 ± 1.7)(4.5 ± 0.5)
CD154b0.7 ± 0.30.5 ± 0.25.3 ± 2.112.9 ± 8.917.8 ± 0.87.5 ± 1.4
(CD40L)b(3.5 ± 0.3)(4.8 ± 0.1)(56.2 ± 101.2)(36.7 ± 58.8)(16.3 ± 3.9)(6.5 ± 1.1)
CD2895.8 ± 1.198.7 ± 1.291.2 ± 6.194.7 ± 5.193.9 ± 4.794.9 ± 4.8
(188 ± 4.2)(552 ± 21.0)(455.2 ± 10.0)(173.3 ± 9.1)(282 ± 21)(375 ± 28)
Chemokine receptors (2° LT and homeostatic)CCR795.1 ± 1.669.0 ± 13.295.5 ± 2.179.0 ± 1.098.1 ± 0.557.7 ± 4.8
(471.8 ± 108.1)(262.9 ± 45.1)(197.2 ± 73.3)(172.8 ± 37.6)(492 ± 61)(209 ± 19)
CXCR498.0 ± 0.794.5 ± 0.693.3 ± 1.968.3 ± 7.390.4 ± 2.860.7 ± 10.0
(574.1 ± 120.0)(558.3 ± 122.8)(215.9 ± 88.8)(215.2 ± 149.3)(191 ± 40)(95.5 ± 46.7)
CCR943.7 ± 3.517.9 ± 1.313.7 ± 1.09.3 ± 3.05.0 ± 1.12.6 ± 0.3
(66.4 ± 8.4)(28.3 ± 2.7)(24.9 ± 3.8)(15.8 ± 4.4)(27.0 ± 12.9)(15.4 ± 1.0)
Chemokine receptors (non-LT and inflammatory)CCR22.8 ± 1.18.5 ± 3.52.4 ± 2.16.0 ± 1.66.7 ± 3.013.7 ± 1.1
(6.9 ± 0.6)(16.8 ± 5.4)(15.5 ± 11.1)(11.0 ± 2.4)(15.0 ± 5.8)(30.3 ± 4.9)
CCR44.8 ± 0.850.9 ± 13.117.3 ± 1.894.2 ± 0.613.4 ± 3.285.4 ± 4.1
(41.1 ± 8.8)(361.1 ± 88.0)(27.0 ± 4.9)(365.1 ± 45.3)(95.7 ± 33.4)(419 ± 88)
CCR50.9 ± 0.420.1 ± 6.16.0 ± 3.253.7 ± 5.36.6 ± 2.643.0 ± 6.2
(6.9 ± 0.4)(31.1 ± 11.3)(14.2 ± 5.9)(84.9 ± 7.4)(11.8 ± 8.0)(34.0 ± 10.6)
CCR62.8 ± 0.929.3 ± 10.97.4 ± 3.954.0 ± 3.921.4 ± 5.274.8 ± 4.2
(13.3 ± 2.1)(65.8 ± 12.6)(18.0 ± 6.4)(86.7 ± 0.9)(74.6 ± 25.0)(137 ± 16.0)
CXCR33.1 ± 0.69.6 ± 3.820.3 ± 8.350.3 ± 2.625.5 ± 6.539.5 ± 6.0
(19.2 ± 8.6)(29.5 ± 2.6)(67.9 ± 29.5)(119.8 ± 20.6)(97.4 ± 72.2)(40.8 ± 44.5)
B follicle and othersCXCR52.1 ± 0.73.2 ± 1.319.4 ± 4.550.9 ± 7.634.1 ± 9.812.9 ± 2.1
(6.7 ± 0.5)(7.0 ± 0.3)(18.5 ± 3.1)(42.7 ± 9.4)(68.8 ± 41.2)(11.7 ± 3.3)
CXCR61.5 ± 0.28.3 ± 4.53.1 ± 1.854.8 ± 2.34.2 ± 1.929.6 ± 7.5
(5.0 ± 0.2)(18.0 ± 6.8)(10.2 ± 4.9)(155.5 ± 6.5)(6.1 ± 3.0)(37.5 ± 4.2)
  • a Averages and SEM of percent cells expressing each Ag are shown along with mean fluorescent intensity in parentheses.

  • b , Intracellular expression.