Table III.

isoAsp content is elevated in spontaneously activated T cells from MRL micea

CD4+ T Cell SubsetsPercentages (±SD)isoAsp Residue (pmol/mg protein ± SD)
B10.BR mouse strain (10-wk-old)MRL/1pr mouse strain (10-wk-old)B10.BR mouse strain (10-wk-old)MRL/lpr mouse strain (10-wk-old)
Naive77.9 ± 3.7%23.5 ± 7.8%71.75 ± 2.8968.75 ± 2.19
Activated3.5 ± 0.1%28.1 ± 2.3%ND102.95 ± 14.21
Memory10.9 ± 0.9%42.7 ± 5.6%ND79.17 ± 0.24
  • a Cell percentages were obtained via FACS analysis for the naive (CD44low CD62Lhigh), activated (CD44highCD62Lhigh), and memory (CD44highCD62Llow) subsets as shown in Fig. 5. After sorting by the expression profile of CD44 and CD62L, cell lysates from naive, activated, and memory CD4+ T cells were measured for intracellular isoAsp content. n = 3.