Table I.

Sequences and characteristics of PCR primers used for the murine genes indicated

PrimersSequencetmCycles
Actin FTCA GAA GGA CTC CTA TGT GG5625
Actin RTCT CTT TGA TGT CAC GCA CG5625
Hsp70 FTCG TGA TCC AGG TGT ACG AG5625
Hsp70 RCGT TGG TGA TGG TGA TCT TG5625
Lmp2 FCTT GCT GCT TCT GTG TCT CG5735
Lmp2 RCAC TGC CAT GAT GGT TGT CT5735
Lmp7 FGCC ACT GCA GGG AGT TAC AT5735
Lmp7 RATC ACC CCG TAG GCA TAG GT5735
Mecl1 FGAA CTG TCA GAG GAA TGC GT5635
Mecl1 RTCA CAC AGG CAT CCA CAT TG5635
Delta FTTG ACC CAG TGG GCT CTT AC5727
Delta RCTT GTG CCA CAG CTC CAA TA5727
X FATC GAA ATG CTT CAC GGA AC5625
X RCTG TTC CCC TCG CTG TCT AC5625
Y FGTT GGA GAG GCT GGA GAC AC5625
Y RAGG TGG GCA GAT TCA AGA TG5625
  • tm, Melting temperature.