Table I.

Sequences of primers and probes used for real-time quantitative PCRa

GeneForward Primer (5′-3′)TaqMan ProbeReverse Primer (5′-3′)
TLR1CCC ATT CCG CAG TAC TCC AT[5′FAM] AGC TCA AAA GTC TCA TGG CCA GGA GGA [3′TAM RA]TTT TCC TTG GGC CAT TCC A
TLR2CCC ATT GCT CTT TCA CTG CT[5′FAM] GTA GTT GTG GGT TGA AGC ACT GGA CAA T [3′TAM RA]CTT CCT TGG AGA GGC TGA TG
TLR3TGG TTG GGC CAC CTA GAA GTA[5′FAM] ACC TGG GCC TTA ATG AAA TTG GGC AA [3′TAM RA]TCT CCA TTC CTG GCC TGT G
TLR4CTG CAA TGG ATC AAG GAC CA[5′FAM] AGG CAG CTC TTG GTG GAA GTT GAA CG [3′TAM RA]TTA TCT GAA GGT GTT GCA CAT TCC
TLR5TGC CTT GAA GCC TTC AGT TAT G[5′HEX] CCA GGG CAG GTG CTT ATC TGA CCT TAA CA [3′TAM RA]CCA CCA CCA TGA TGA GAG CA
TLR6CCC TCA ACC ACA TAG AAA CG[5′HEX] ACC GAC TTG GAA ATG CCT GGT CAG [3′TAM RA]GAG ATA TTC CAC AGG TTT GG
TLR7TTA CCT GGA TGG AAA CCA GCT ACT[5′HEX] AGA TAC CGC AGG GCC TCC CGC [3′TAM RA]TCA AGG CTG AGA AGC TGT AAG CTA
TLR8AAC TTT CTA TGA TGC TTA CAT TTC TTA TGA C[5′HEX] CCA AAG ATG CCT CTG TTA CTG ACT GGG TG [3′TAM RA]GGT GGT AGC GCA GCT CAT TT
TLR9TGA AGA CTT CAG GCC CAA CTG[5′FAM] AGC ACC CTC AAC TTC ACC TTG GAT CTG TC [3′ TAM RA]TGC ACG GTC ACC AGG TTG T
TLR10TTT GAT CTG CCC TGG TAT CTC A[5′HEX] AGG TCA ATG CAC ACA AAC ATG GCA CA [3′TAM RA]AGT TGT TCT TGG GTT GTT TTC CTA AC
CD4 promoterTTC CAC ACT GGG CCA CCT AT[5′FAM] CAC TGG ACA CAA TTG CCC TCA GG [3′TAM RA]TTG TGG GCT TAC CAC TGC TG
  • a The quencher dye, TAMRA, was placed at the 3′ end of probes. The reporter dyes, linked to the 5′ end, were HEX, FAM, or VIC (18S ribosomal RNA).