Table I.

HHV-8 infection of primary cells and cell lines expressing DC-SIGN

Cell typeDC-SIGN Expressionaα3β1 ExpressionaK8.1 ExpressionbORF 59 Expressionb
UntreatedAnti-DC-SIGN treatedUntreatedAnti-DC-SIGN Treated
DCs92 (87–97) n = 776 (72–80) n = 250 (20–80) n = 84 (0–10) n = 875 (68–100) n = 87 (3–12) n = 8
Macrophages5 (2–10) n = 471 (40–75) n = 48 (6–10) n = 40 (0–3) n = 48 (0–16) n = 40 (0–3) n = 4
IL-13 Macrophages74 (50–80) n = 478 (62–95) n = 463 (60–66) n = 410 (0–21) n = 488 (66–100) n = 40 (0–0) n = 4
B-LCL0 (0–0) n = 51 (0–2) n = 30 (0–0) n = 50 (0–0) n = 30 (0–0) n = 50 (0–0) n = 3
B-LCL-DC-SIGN73 (49–85) n = 51 (0–2) n = 391 (98–100) n = 50 (0–3.3) n = 388 (83–100) n = 50 (0–0) n = 3
K5620 (0–0) n = 50 (0–0) n = 20 (0–0) n = 5ndndcnd
K562-DC-SIGN68 (55–75) n = 40 (0–0) n = 288 (72–90) n = 5ndndnd
  • a DC-SIGN or α3β1 integrin expression on the surface of cells as measured by FACS and expressed as percent of positive or negative cells for fluorescence after staining with mAb specific for DC-SIGN and α3β1 as compared to the isotype control or mouse anti-IgG FITC alone. n, Number of determinations. Data are expressed as median (range).

  • b Percent of cells reacting positive with mAb against K8.1A/B or ORF59 at 24 h postinfection. Data are expressed as median (range). n, Number of determinations.

  • c nd, Not done.