Cell type | DC-SIGN Expression^{a} | α_{3}β_{1} Expression^{a} | K8.1 Expression^{b} | ORF 59 Expression^{b} | ||
---|---|---|---|---|---|---|
Untreated | Anti-DC-SIGN treated | Untreated | Anti-DC-SIGN Treated | |||
DCs | 92 (87–97) n = 7 | 76 (72–80) n = 2 | 50 (20–80) n = 8 | 4 (0–10) n = 8 | 75 (68–100) n = 8 | 7 (3–12) n = 8 |
Macrophages | 5 (2–10) n = 4 | 71 (40–75) n = 4 | 8 (6–10) n = 4 | 0 (0–3) n = 4 | 8 (0–16) n = 4 | 0 (0–3) n = 4 |
IL-13 Macrophages | 74 (50–80) n = 4 | 78 (62–95) n = 4 | 63 (60–66) n = 4 | 10 (0–21) n = 4 | 88 (66–100) n = 4 | 0 (0–0) n = 4 |
B-LCL | 0 (0–0) n = 5 | 1 (0–2) n = 3 | 0 (0–0) n = 5 | 0 (0–0) n = 3 | 0 (0–0) n = 5 | 0 (0–0) n = 3 |
B-LCL-DC-SIGN | 73 (49–85) n = 5 | 1 (0–2) n = 3 | 91 (98–100) n = 5 | 0 (0–3.3) n = 3 | 88 (83–100) n = 5 | 0 (0–0) n = 3 |
K562 | 0 (0–0) n = 5 | 0 (0–0) n = 2 | 0 (0–0) n = 5 | nd | nd^{c} | nd |
K562-DC-SIGN | 68 (55–75) n = 4 | 0 (0–0) n = 2 | 88 (72–90) n = 5 | nd | nd | nd |
a DC-SIGN or α_{3}β_{1} integrin expression on the surface of cells as measured by FACS and expressed as percent of positive or negative cells for fluorescence after staining with mAb specific for DC-SIGN and α_{3}β_{1} as compared to the isotype control or mouse anti-IgG FITC alone. n, Number of determinations. Data are expressed as median (range).
b Percent of cells reacting positive with mAb against K8.1A/B or ORF59 at 24 h postinfection. Data are expressed as median (range). n, Number of determinations.
c nd, Not done.