Table II.

Macroarray analysis of polarized Caco-2 cell gene expression during Shigella infectiona

Probe SetNI (mean ± SD)M90T (mean ± SD)P valueFold Change
Vimentin14.62 ± 0.1814.15 ± 0.130.041027−1.39
Postmeiotic segregation 114.32 ± 0.0913.97 ± 0.090.008549−1.27
Human chemokine α 3 (CKA-3)13.44 ± 0.113.1 ± 0.090.015804−1.27
Collagen, type II, α 114.23 ± 0.1213.91 ± 0.090.038098−1.25
PAC 179D313.8 ± 0.0813.55 ± 0.050.009966−1.20
Uk-1313.59 ± 0.0513.39 ± 0.060.015796−1.16
v-erb-a avian erythroblastic leukemia viral oncogene13.35 ± 0.0913.14 ± 0.030.045152−1.16
Bullous pemphigoid antigen 113.25 ± 0.0613.05 ± 0.050.014533−1.15
FK506-binding protein 1A (12 kDa)13.75 ± 0.0713.55 ± 0.060.043947−1.15
Flavin containing monooxygenase 413.11 ± 0.0312.95 ± 0.040.004456−1.13
FK506-binding protein 4 (59 kDa)13.36 ± 0.0613.19 ± 0.040.031667−1.12
Bcl-2 binding protein13.35 ± 0.0513.2 ± 0.030.012662−1.11
Heparan sulfate proteoglycan 213.15 ± 0.0513.02 ± 0.050.041943−1.10
IL-213.09 ± 0.0913.32 ± 0.040.0236951.18
CXCL113.09 ± 0.0813.33 ± 0.080.0421541.19
IEX13.21 ± 0.0513.45 ± 0.080.0164121.19
Connective tissue growth factor13.05 ± 0.0513.36 ± 0.070.0008671.24
CXCL312.9 ± 0.0713.22 ± 0.120.0370181.24
Coproporphyrinogen oxidase14.19 ± 0.0814.53 ± 0.10.012931.27
SOCS-313.34 ± 0.1213.68 ± 0.10.0322771.27
CL100 mRNA for protein tyrosine phosphatase12.9 ± 0.0713.24 ± 0.080.0032151.27
IRF-112.99 ± 0.0613.37 ± 0.140.0198691.30
Ribosomal protein S317.22 ± 0.1117.63 ± 0.090.0096221.32
Ryudocan13.99 ± 0.0714.4 ± 0.140.021051.32
MAD-3 (IK-B like activity)12.95 ± 0.0713.37 ± 0.130.0072911.34
CCL20 (MIP-3 α)13.61 ± 0.1214.1 ± 0.180.0324251.39
GRO-2 oncogene12.91 ± 0.113.42 ± 0.180.0188731.42
Heat shock 70 KDa protein 614.09 ± 0.1214.61 ± 0.120.0055951.42
Homo sapiens chemokine exodus-112.73 ± 0.113.27 ± 0.220.037421.45
TNF ip2013.16 ± 0.0313.74 ± 0.140.0009841.49
Amphiregulin13.92 ± 0.1614.52 ± 0.10.0034591.52
CXCL213.37 ± 0.0614.01 ± 0.230.0153171.56
EST-214.46 ± 0.215.11 ± 0.140.0110171.58
v-jun13.4 ± 0.1214.14 ± 0.130.0003431.67
Superoxide dismutase 2, mitochondrial16.83 ± 0.2517.66 ± 0.30.0411351.77
Heat shock 70 KDa protein 1A15.12 ± 0.1816.28 ± 0.120.0000132.23
  • a Signal intensity resulting from macroarray hybridizations done with eight biological replicates of M90T infected Caco-2 cells were normalized and log2 transformed. Data analysis using the unpaired Welch t test from the dChip software gives a fold change between experimental point and baseline in a linear scale and an associated p value. Results with a p < 0.05 were considered as statistically significant.