Table VIII.

Binding of Hu1D10 Abs to protein Ga

Hu1D10nIC50 (μg/ml)Relative Binding
IgG1 WT314.3 ± 2.5
IgG1 T250Q/M428L316.5 ± 4.50.87
IgG2M3 WT311.7 ± 1.2
IgG2M3 T250Q/M428L316.3 ± 2.20.72
IgG4 WT319.6 ± 1.0
IgG4 T250Q/M428L321.5 ± 7.70.91
  • a Competitive binding experiments were done by ELISA as described in Materials and Methods. The mean IC50 ± SD are reported for three independent experiments. The relative improvement in binding for each mutant was calculated by comparison to the corresponding WT.