Table III.

Binding of OST577 Abs to rhesus FcRna

OST577nIC50 (μg/ml)Relative Binding
IgG1 WT38.86 ± 0.52
IgG1 T250Q32.97 ± 0.593.0
IgG1 M428L30.629 ± 0.06014
IgG1 T250Q/M428L30.236 ± 0.01337
  • a Competitive binding experiments were done by flow cytometry as described in Materials and Methods. The mean IC50 ± SD are reported for three independent experiments. The relative improvement in binding for each mutant was calculated by comparison to the WT.