Table I.

Binding of OST577 Abs to HBsAga

OST577nIC50 (μg/ml)Relative Binding
IgG1 WT30.949 ± 0.059
IgG1 T250Q31.09 ± 0.070.87
IgG1 M428L30.940 ± 0.0691.0
IgG1 T250Q/M428L31.20 ± 0.130.80
  • a Competitive binding experiments were done by ELISA as described in Materials and Methods. The mean IC50 ± SD are reported for three independent experiments. The relative binding of each mutant was calculated by comparison to the WT.