Table V.

Pretreatment of mice with Ft LVS LPS i.p. blunts up-regulation of proinflammatory genes in response to Ft LVS infectiona

Day−2−2−7−7−7−7
PretreatmentFt LVS LPSFt LVS LPSFt LVS LPSFt LVS LPSSalineSaline
day 0 treatmentsalineFt LVSsalineFt LVSsalineFt LVS
ProinflammatoryTNF-αb116117137
IL-12 p35211116
IL-12 p401412121
IL-1β141618
IL-61313212
IFNIFN-β113312
IFN-γ11551111765
ChemokinesIP-101441571133
KC114130169
MCP-11211311107
RANTES111113
Anti-inflammatoryIL-4111111
IL-10111112
OthersiNOS1991701535
  • a Groups of five mice were pretreated with saline or 100 ng of Ft LVS LPS 2 or 7 days before challenge with Ft LVS on day 0. Two days after challenge, mice were sacrificed, and RNA extracted from livers was analyzed for expression of the indicated genes. Results are expressed as mean fold induction above levels detected in livers of mice injected with saline only.

  • b Gene expression data for IL-12 p35, IL-12 p40, iNOS, TNF-α, IP-10, and IFN-γ are the same data depicted in Fig. 6B.