Table I.

CCL18 is secreted by Der p 1-stimulated circulating pDC from AA patientsa

DonorsCellsMediumDer p 1IL-4
AApDC6.5 ± 3.0199.9 ± 182b48.2 ± 28.9
mDC7.8 ± 3.317.4 ± 6.750.6 ± 34.9
Monocytes 24 h1.8 ± 1.82.1 ± 2.160.9 ± 25.5b
Monocytes 48 h22.1 ± 14.26.2 ± 3.6217.2 ± 101.4b
Monocytes 72 h18.8 ± 7.619.4 ± 5.2586.2 ± 199.6b
NApDC2.95 ± 1.93.1 ± 2.030.9 ± 17.8
mDC6.9 ± 3.83.1 ± 2.031.1 ± 12.5
Monocytes 24 h2.7 ± 1.70113.7 ± 36.3b
Monocytes 48 h6.7 ± 2.40597.6 ± 315.4b
Monocytes 72 h33.9 ± 10.535.6 ± 14.23366.8 ± 1527b
  • a pDC and mDC were isolated from six NA and six AA donors, and monocytes were isolated from seven NA and seven AA donors. DC were cultured for 24 h, monocytes for 24, 48, and 72 h with or without Der p 1 (500 ng/ml) or IL-4 (10 ng/ml). CCL18 concentration was evaluated by ELISA. Results are expressed as mean pg/ml ± SEM;

  • b p < 0.05 compared to medium.