Table III.

Characterization of neutralization epitopes with non-natural RBD mutants by ELISAa

GroupMAbDeletion ofSingle-Point Mutation
aa 318–326aa 491–510K390AD429AR441AE452AD454AD463A
Conf I9F70.080.00 2.15 0.180.02 1.36 0.01 1.47
10E 70.110.00 2.39 0.190.01 1.44 0.03 1.71
12B110.030.00 2.40 0.090.030.190.00 0.45
18C20.030.01 2.45 0.120.010.030.00 0.34
24H80.07−0.01 2.32 0.190.02 0.29 0.00 1.35
29G20.060.00 2.41 0.170.01 0.71 0.02 1.37
32H50.080.00 2.51 0.200.01 0.82 0.01 1.46
Conf II20E 70.02−0.01 2.36 0.120.02 0.65 0.00 1.24
26A40.020.00 2.42 0.140.02 0.65 0.00 1.08
27C10.070.03 2.36 0.190.12 0.79 0.01 1.32
31H120.080.00 2.48 0.200.01 1.03 0.00 1.87
34E 100.08−0.01 2.74 0.200.01 1.02 0.01 1.72
Conf III11E 120.010.00 2.05 0.010.02 0.25 0.010.05
18D90.010.00 2.18 0.010.02 0.39 0.00 0.44
Conf IV28D60.070.00 2.37 0.070.02 1.69 0.00 1.49
30F90.060.00 2.43 0.050.02 1.59 0.00 1.37
35B50.08−0.01 2.48 0.110.02 1.65 0.01 1.57
Conf V24F4 1.73 1.22 2.75 1.45 0.73 2.59 1.95 0.86
33G4 2.03 1.56 2.50 1.71 1.05 2.56 2.06 2.02
38D4 1.27 1.06 2.49 0.77 0.50 2.41 1.30 0.77
Conf VI13B60.00−0.01 2.32 0.150.02 1.40 0.00 1.28
19B20.04−0.01 2.36 0.000.01 1.15 0.00 0.86
44B50.130.00 2.41 0.200.03 1.70 0.04 1.99
45F60.080.00 2.43 0.200.02 1.78 0.03 1.62
Linear4D5 2.70 2.20 1.75 0.89 0.83 3.03 2.44 0.82
17H9 2.95 3.62 2.63 2.78 3.59 3.23 0.19 4.18
Control3A30.010.020.020.000.030.020.010.03
  • a Ags were coated to ELISA plates at 1 μg/ml, and mAbs were tested at 10 μg/ml. OD450 value >0.2 was considered positive reaction and highlighted in boldface.