Table I.

IL-17 selectively enhances the production of angiogenic CXC chemokines by human NSCLC cellsa

IL-17Sq-19A549LK-87
+++
aFGF21.6 ± 0.522.4 ± 3.620.8 ± 0.917.7 ± 0.520 ± 2.021.9 ± 2.4
Basic FGF<10<1047 ± 350 ± 317 ± 118 ± 1
Angiopoietin-2132 ± 4140 ± 21157 ± 7147 ± 11133 ± 18123 ± 11
TGF-α8.9 ± 1.49.3 ± 2.113.9 ± 0.713.3 ± 0.723.7 ± 3.925.5 ± 4.3
HGF150 ± 9147 ± 14178 ± 10162 ± 17135 ± 8137 ± 6
CXCL10.9 ± 0.12.1 ± 0.1b0.6 ± 0.12.5 ± 0.1b0.9 ± 0.12.5 ± 0.3b
CXCL51.7 ± 0.17.3 ± 0.6b10.6 ± 0.714.8 ± 1.1b13.7 ± 0.519.2 ± 1.9b
CXCL63.6 ± 0.774.7 ± 10.5b50.8 ± 7.091.7 ± 22.6b23.0 ± 7.760.2 ± 6.0b
CXCL82.1 ± 0.14.2 ± 0.1b0.15 ± 0.020.52 ± 0.05b0.23 ± 0.030.43 ± 0.03b
CXCL1010.7 ± 1.111.1 ± 0.737.7 ± 2.740.7 ± 3.717.1 ± 0.517.7 ± 1.5
CXCL11<62.5<62.5<62.5<62.5<62.5<62.5
CXCL1279 ± 1277 ± 3793 ± 3091 ± 564 ± 367 ± 5
PDGF-BB367 ± 21360 ± 8337 ± 5334 ± 7330 ± 23335 ± 7
PGE24 ± 15 ± 165 ± 463 ± 7108 ± 5111 ± 7
VEGF-A1.4 ± 0.11.3 ± 0.10.65 ± 0.130.66 ± 0.150.9 ± 0.11.0 ± 0.1
  • a Cells were cultured in RPMI 1640 containing 5% FCS, 2 mM l-glutamine, 0.1 mM NEAA, 100 IU/ml penicillin, and 100 μg/ml streptomycin with or without 50 ng/ml IL-17 for 48 h. Cell-free supernatants were collected and stored at −70°C. Concentrations of cytokines were measured using commercially available ELISA kits. PGE2 concentration was measured as reported previously (36 ). (pg/ml for aFGF, bFGF, angiopoietin-2, TGF-α, HGF, CXCL6, CXCL10, CXCL11, CXCL12, PDGF-BB, and PGE2; ng/ml for CXCL1, CXCL5, CXCL8, and VEGF-A).

  • b , p < 0.05.