Table III.

VRT101 peptide binding and renal pathogenicity of anti-DNA mAbsa

mAbsAnti-DNA Abs (cpm)Anti-VRT101 Abs (OD 405 nm)Pathogenicityb
A5217521.31+3
C7216371.25+3
3F714001.4+3
1D917841.08+2
J3816000.52
11C418000.03+/−c
J6420380.08
  • a Cells (106) of different anti-DNA-producing hybridoma lines (originating from (NZB × NZW) F1 mice) were inoculated in 1 to 8-wk-old RAG-1-deficient mice (six mice per group). Sera were tested for DNA (by RIA) and VRT101 (by ELISA) binding and kidney pathology was evaluated as detailed in Materials and Methods. Results are the mean of duplicates (ELISA) and triplicates (RIA).

  • b Pathogenicity was evaluated in all five mice of each group. All of the pathogenic Abs that bound the kidneys showed a similar pattern of pathology and were also found to induce proteinuria in these mice. The pathogenic Abs produced an intense linear deposition along the glomerular capillary wall. The kidney pathology was expressed as follows: +3, severe diffuse GN; +2, moderate diffuse GN; +1, mild GN; and +/−, borderline GN.

  • c 1IC4 Ab induced mostly mesangial deposition with little or no structural changes observed by light microscopy.