Table V.

Structural and phylogenetic characteristics of VACWR immunogenic Ags

VACV-WR Gene NameAg NameaProtein SizeTime of ExpressionDescriptionFunction CategoryEpitopeRestriction Element% of Similarity with VAR-Majorb% of Similarity with MVAb
VACWR063E7R166LateSoluble, myristylprotein has N-myristyltransferase targetE7R (130–138)HLA-A*110188.8 (E7R-VAR-IND)100 (MVA054R)
VACWR094J2R177EarlyThymidine kinaseViral regulationJ2R (116–124)HLA-B*070288.8 (L2R)100 (MVA086R)
VACWR106D1R844EarlyLarge subunit of mRNA capping enzymeViral regulationD1R (808–817)HLA-B*0702100 (F1R)90 (MVA098R)
VACWR157A34R168LateEEV glycoproteinVirion structureA34R (82–90)HLA-B*0702100 (A37R)100 (MVA145R)
VACWR172A46R240Toll/IL1-receptor [TIR]-likeVirulence factorA46R (142–150)HLA-A*0201100 (A52R-VAR-IND; A49R-VAR-BSH)100 (MVA159R)
VACWR188B6R173Ankyrin-likeB6R (108–116)HLA-A*020155.5 (A2L)100 (MVA174R)
VACWR195B14R345EarlySerpin, SPI-2/CrmAVirulence factorB14R (327–335)HLA-A*0201100 (B13R-VAR-IND; B12R-VAR-BSH)55.5 (MVA025L)
VACWR030M1L472EarlyAnkyrin-likeM1L (374–383)HLA-A*0201100 (O1L)≤50
VACWR070I1L312LateVirion core DNAbinding proteinViral regulationI1L (211–219)HLA-A*0201100 (K1L)100 (MVA062L)
VACWR072I3L269EarlyssDNA-binding phosphoproteinViral regulationI3L (116–124)HLA-A*1101100 (K3L)100 (MVA064L)
VACWR085G7L371LateIMV protein, proteolytic processVirion structureG7L (250–258)HLA-A*0201100 (H7L)100 (MVA077L)
VACWR117D12L287EarlySmall subunit of mRNA capping enzymeViral regulationD12L (251–259)HLA-A*0201100 (N2L)100 (MVA109L)
VACWR125A6L372A6L (6–14)HLA-A*0201100 (A6L-VAR-IND; A7L-VAR-BSH)100 (MVA117L)
VACWR129A10L891LatePrecursor p4a of core protein 4aVirion structureA10L (171–180)HLA-A*1101100 (A10L-VAR-IND; A11L-VAR-BSH)100 (MVA121L)
VACWR133A14L90LateIMV membrane proteinVirion structureA14L (51–59)HLA-A*0201100 (A14L-VAR-IND; A15L-VAR-BSH)100 (MVA125L)
VACWR137A17L203LateIMV surface membrane proteinVirion structureA17L (61–70)HLA-A*0201100 (A17L-VAR-IND; A18L-VAR-BSH)100 (MVA128L)
VACWR137A17L203LateIMV surface membrane proteinVirion structureA17L (81–90)HLA-A*0201100 (A17L-VAR-IND; A18L-VAR-BSH)100 (MVA128L)
VACWR173A47L252A47L (139–148)HLA-A*1101100 (J1L)100 (MVA160L)
VACWR082434VACWR082 (18–26)HLA-A*0201100 (H5R)100 (MVA074R)
VACWR050348VACWR050 (196–204)HLA-A*0201100 (C15L)55.5 (MVA056L)
VACWR101H3L324LateIMV heparin binding surface proteinVirion structureH3L (184–192)HLA-A*0201100 (I3L)100 (MVA093L)
  • a Name from VACV-Cop. GenBank accession no. M35027.

  • b Percentage similarity of the determinant.

  • cPercentage of similarity with ACAM3000 MVA176 (AAT10572 in GenBank protein database).