Table III.

Immunogenicity of vaccinia virus-derived HLA-B*0702-restricted peptides in different mouse strains

HLA-B7 Potential EpitopesHLA-B7/Kb (CD8+/LPS blasts) (n = 2)Av. Net SFCs/106 Cellsa
PeptideSequence% of peptide responseAv. net SFCs/106 cellsaCB6F1 (CD8+/LPS blasts) (n = 2)Kb−/−Db−/− (CD8+/LPS blasts) (n = 2)CBA/J (CD8+/LPS blasts) (n = 2)
A34R (82–90)LPRPDTRHL75285198
J2R (116–124)KPFNNILNL1766b
D1R (808–817)RPSTRNFFEL728
Vaccinia-infected target cellsNA367110043820
  • a Net SFCs/106 cells are calculated according to the formula: [(number of spots against relevant peptides − (number of spots against irrelevant peptides (HCV core 132)] × [(1 × 106)/number of effector cells/well] (HBV envelope 378).

  • b (-) indicates <20 SFCs/106 cells. Control irrelevant peptide were on average 6 SFCs/106 cells for CB6F1, 5 for HLA-B7/Kb, 2 for Kb−/−Db−/−, and 90 for CBA/J.